Investigating the effects of heat shock, osmotic stress and drying methods on the viability of safflower primed seeds during deterioration period

Document Type : scientific research article

Authors

1 Ph.D. Graduate of Crop Physiology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Professor, Dept. of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Professor, Dept. of Biology, Golestan University, Gorgan, Iran

Abstract

Background and purpose: In recent decades, seed priming has become a popular technique for enhancing germination rate and uniformity in the field, as well as promoting better seedling establishment and faster adaptation to adverse environmental conditions during germination and seedling growth. However, the practical application of this method has been limited due to the decline in seed quality and longevity associated with priming. Thus, this study aims to investigate the impact of post-priming treatments, such as heat shock, osmotic stress, and drying methods, on the longevity of safflower seeds under various deterioration periods.

Materials and methods: In this study, the seeds of the Sina cultivar were used. First, the seeds were immersed in water and salicylic acid solution. After the completion of the imbibed phase, they were subjected to heat shock treatments (1.5, 3 and 8 hours at 45 °C), osmotic stress (-1.5 and -2.5 MPa applied by polyethylene glycol), and two slow and fast drying methods. The treatment without priming was also considered as a control. Finally, the seeds were subjected to controlled deterioration for 0, 2, 4, and 6 days at 45 °C.

Results: The results indicated that as the duration of deterioration increased, the germination ability and seedling growth decreased. In the treatment where deterioration lasted for 6 days, the seeds died completely. The decline in quality of primed seeds was more severe under the conditions of heat shock and osmotic stress after priming, compared to the control seeds. However, heat shock (8 hours) resulted in improved quality of the primed seeds compared to shorter heat shock durations. The primed seeds with salicylic acid and water (hydro) showed f 13% and 10% increase compared to the control treatment, respectively. Applying drying treatments after priming, without the use of heat shock and osmotic stress treatments, resulted in maintaining and even increasing the germination and seedling growth of primed seeds compared to unprimed seeds at various levels of deterioration. Both drying methods improved the germination and seedling growth of primed seeds, resulting in greater germination and seedling growth compared to non-primed seeds.

Conclusion: In general, it is advisable to employ an appropriate method to dry primed safflower seeds in order to maintain or enhance their longevity during the priming process and after the completion of the soaking period.

Conclusion: In general, it is advisable to employ an appropriate method to dry primed safflower seeds in order to maintain or enhance their longevity during the priming process and after the completion of the soaking period.

Keywords

Main Subjects

References

1.Maghsoudi, E., Yadavi, A., Balouchi, H., Dehnavi, M. M., Piri, R., & Mastinu, A. (2024). Improving the physiological properties and yield of safflower by combining organic and chemical nitrogen in different irrigation cut-off conditions. Industrial Crops and Products, 222, 1-13.
2.Nile, S. H., Thiruvengadam, M., Wang, Y., Samynathan, R., Shariati, M. A., Rebezov, M., Nile, A., Sun, M., Venkidasamy, B., Xiao, J., & Kai, G. (2022). Nano-priming as emerging seed priming technology for sustainable agriculture-recent developments and future perspectives. Journal of Nanobiotechnology, 20(1), 1-31.
3.Thakur, M., Tiwari, S., Kataria, S., & Anand, A. (2022). Recent advances in seed priming strategies for enhancing planting value of vegetable seeds. Scientia Horticulturae, 305, p.111355.
4.Fallah, S., Malekzadeh, S., & Pessarakli, M. (2018). Seed priming improves seedling emergence and reduces oxidative stress in Nigella sativa under soil moisture stress. Journal of Plant Nutrition, 41(1), 29-40.
5.Silva, B. N. P., Masetto, T. E., & Rocha, L. G. (2024). An insight into seed priming response of Crotalaria ochroleuca and Crotalaria spectabilis during storage. Brazilian Journal of Biology, 84, p.e279806.
6.Tu, K., Cheng, Y., Pan, T., Wang, J., & Sun, Q. (2022). Effects of seed priming on vitality and preservation of pepper seeds. Agriculture, 12(5), p.603.
7.Pagano, A., Macovei, A., & Balestrazzi, A. (2023). Molecular dynamics of seed priming at the crossroads between basic and applied research. Plant Cell Reports, 42(4), 657-688.
8.Ren, M., Tan, B., Xu, J., Yang, Z., Zheng, H., Tang, Q., Zhang, X., & Wang, W. (2023). Priming methods affected deterioration speed of primed rice seeds by regulating reactive oxygen species accumulation, seed respiration and starch degradation. Frontiers in Plant Science, 14, p.1267103.
9.Sano, N., & Seo, M. (2019). Cell cycle inhibitors improve seed storability after priming treatments. Journal of Plant Research, 132, 263-271.
10.Sibande, G. A. K., Kabambe, V. H., Maliro, M. F. A., & Karoshi, V. (2015). Effect of priming techniques and seed storage period on soybean (Glycine max L.) germination. Journal of Dynamics in Agricultural Research, 2(5), 46-53.
11.Batista, T. B., Fernandez, G. J., Da Silva, T. A., Maia, J., & Da Silva, E. A. A. (2020). Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment. AoB Plants, 12 (5) [online].
12.Piri, R., Moradi, A., & Hosseini-Moghaddam, M. (2018). Effect of accelerated aging and seed priming on germination and some biochemical indices of cumin (Cuminum cyminum L.). Iranian Journal of Seed Science and Research, 5(1), 69-81.
13.Singh, N., Bhuker, A., Pandey, V., Punia, H., Sourabh, Singh, B., Ahmad, A., Tyagi, A., & Malik, A. (2024). Nano-enhanced storage of American cotton using metal-oxide nanoparticles for improving seed quality traits. Scientific Reports, 14(1), 24445.
14.Bore, E. K., Ishikawa, E., Libron, J. A. M. A., Goto, K., Odama, E., Nakao, Y., Yabuta, S., & Sakagami, J. I. (2023). Primed seeds of NERICA 4 stored for long periods under high temperature and humidity conditions maintain germination rates. Applied Sciences, 13(5), 2869.
15.Bora, P. P., Barua, M., Deka, S.D., Das, P., & Barua, P. K. (2023). Influence of seed storage and priming on naturally aged seeds of aromatic Joha Rice. International Journal of Environment and Climate Change, 13(11), 372-383.
16.Ali, N., Rab, A., & Shah, Z. (2013). Role of pre storage seed priming in controlling seed deterioration during storage. Sarhad Journal of Agriculture, 29(3), 379-386.
17.Rasoolzadeh, L., Salehi Shanjani, P., & Jafari, A. A. (2020). Effects of seed priming on germination characteristics of Achillea millefolium seeds under different ageing treatment. Journal of Medicinal plants and By-product, 9(1), 79-89.
18.Kaya, M. D., Ergin, N., Harmancı, P., & Kulan, E. G. (2024). Seed priming as a method of preservation and restoration of sunflower seeds. Oilseeds and fats, Crops and Lipids, 31 (4), 1-7.
19.Jovicic, D., Ovuka, J., Nikolic, Z., Petrovic, G., Marinkovic, D., Stojanovic, M., & Tamindzic, G. (2022). Potential of two hydration treatments for improvement of sunflower seed vigor. Seed Science and Technology, 50(3), 357-366.
20.Panghal, V. P. S., Bhuker, A., Duhan, D. S., & Kumar, A. (2023). Maintaining onion seed quality during storage through seed priming. Agricultural Reviews, 44(2), 269-272.
21.Bruggink, G. T., Ooms, J., & Van der Toorn, P. (1999). Induction of longevity in primed seeds. Seed Science Research, 9(1), 49-53.
22.Adetunji, A. E., Adetunji, T. L., Varghese, B., Sershen, & Pammenter, N. W. (2021). Oxidative stress, ageing and methods of seed invigoration: an overview and perspectives. Agronomy, 11(12), p.2369.
23.Schwember, A. R., & Bradford, K. J. (2005). Drying rates following priming affect temperature sensitivity of germination and longevity of lettuce seeds. HortScience, 40(3), 778-781.
24.Fabrissin, I., Sano, N., Seo, M., & North, H. M. (2021). Ageing beautifully: can the benefits of seed priming be separated from a reduced lifespan trade-off?. Journal of Experimental Botany, 72(7), 2312-2333.
25.Wahid, A., Gelani, S., Ashraf, M., & Foolad, M. R. (2007). Heat tolerance in plants: an overview. Environmental and Experimental Botany, 61(3), 199-223.
26.Gurusinghe, S., Powell, A. L., & Bradford, K. J. (2002). Enhanced expression of BiP is associated with treatments that extend storage longevity of primed tomato seeds. Journal of the American Society for Horticultural Science, 127(4), 528-534.
27.Ansari, O., Sharifzadeh, F., Moradi, A., Azadi, M. S., & Younesi, E. (2013). Heat shock treatment can improve some seed germination indexes and enzyme activity in primed seeds with gibberellin of mountain rye (Secale montanum) under accelerated aging conditions. Cercetari Agronomice in Moldova, 4, 156.
28.Malek, M., Ghaderifar, F. Torabi, B., & Sadeghipour, H. R. (2019). Rapeseed seed viability reaction to priming treatments and drying conditions of primed seeds. Journal of Agronomy, 22(1), 27-4 .[In Persian with English Summary]
29.Ventura, L., Dona, M., Macovei, A., Carbonera, D., Buttafava, A., Mondoni, A., Rossi, G., & Balestrazzi, A.
(2012). Understanding the molecular pathways associated with seed vigor. Plant Physiology and Biochemistry, 60, 196-206.
30.Ratikanta, K. M. (2011). Seed priming: an efficient farmers’ technology to improve seedling vigour, seedling establishment and crop productivity. International Journal of Bio-resource and Stress Management, 2, 297.
31.Ghaderifar, F., & Gorzin, M. (2018). Applied researches in seed technology. Publications of Gorgan University of Agricultural Sciences and Natural Resources, 240 p.
32.Demir, I., Ermis, S., & Okcu, G. (2005). Effect of dehydration temperature and relative humidity after priming on quality of pepper seeds. Seed Science and Technology, 33(3), 563-569.
33.Soeda, Y., Konings, M. C., Vorst, O., Van Houwelingen, A. M., Stoopen, G. M., Maliepaard, C. A., Kodde, J., Bino, R. J., Groot, S. P., & Van Der Geest, A. H. (2005). Gene expression programs during Brassica oleracea seed maturation, osmopriming, and germination are indicators of progression of the germination process and the stress tolerance level. Plant Physiology, 137(1), 354-368.
34.Coolbear, P., Toledo, P., & Seetagoses, U. (1992). Effects of temperature of pre-sowing hydration treatment and subsequent drying rates on the germination performance of celery seed. New Zealand Journal of Crop and Horticultural Science, 19(1), 9-14.
35.Bujalski, W., & Nienow, A. (1991). Large-scale osmotic priming of onion seeds: a comparison of different strategies for oxygenation. Scientia Horticulturae, 46(2), 13-24.
36.ISTA. (2009). International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland, pp: 48.
37.Ghaderi-Far, F., Alimagham, S. M., Kameli, A. M., & Jamali, M. (2012). Isabgol (Plantago ovata Forsk) seed germination and emergence as affected by environmental factors and planting depth. International Journal of Plant Production, 6, 185-194.
38.Li, W., Niu, Y., Zheng, Y., & Wang, Z. (2022). Advances in the understanding of reactive oxygen species-dependent regulation on seed dormancy, germination, and deterioration in crops. Frontiers in Plant Science, 13, 826809.
39.Kumar, A., Nayak, A. K., Hanjagi, P. S., Kumari, K., Vijayakumar, S., Mohanty, S., Tripathi, R., & Panneerselvam, P. (2021). Submergence stress in rice: Adaptive mechanisms, coping strategies and future research needs. Environmental and Experimental Botany, 186, p.104448.
40.Hussain, S., Zheng, M., Khan, F., Khaliq, A., Fahad, S., Peng, S., Huang, J., Cui, K., & Nie, L. (2015). Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Scientific Reports, 5(1), 8101.
41.Lima, J. J. P., Buitink, J., Lalanne, D., Rossi, R. F., Pelletier, S., da Silva, E. A. A., & Leprince, O. (2017). Molecular characterization of the acquisition of longevity during seed maturation in soybean. Plos One, 12(7), 1-25.
42.Waterworth, W. M., Masnavi, G., Bhardwaj, R. M., Jiang, Q., Bray, C. M., & West, C. E. (2010). A plant DNA ligase is an important determinant of seed longevity. The Plant Journal: for Cell and Molecular Biology, 63(5), 848-860.
43.Oge, L., Bourdais, G., Bove, J., Collet, B., Godin, B., Granier, F., Boutin, J. P., Job, D., Jullien, M., & Grappin, P. (2008). Protein repair L-isoaspartyl methyltransferase1 is involved in both seed longevity and germination vigor in Arabidopsis. The Plant Cell, 20(11), 3022-3037.
44.Kaur, H., Petla, B. P., & Majee, M. (2016). Small heat shock proteins: roles in development, desiccation tolerance and seed longevity. In: Asea AAA, Calderwood S.; Kaur, P, eds. Heat shock proteins and plants. New York: Springer, 3-18.
45.Wang, W., He, A., Peng, S., Huang, J., Cui, K., & Nie, L. (2018). The effect of storage condition and duration on the deterioration of primed rice seeds. Frontiers in Plant Science, 9, 1-17.
46.Bewley, J. D., Bradford, K., & Hilhorst, H. (2013). Seeds: physiology of development, germination and dormancy. Springer Science and Business Media. 392 pp.
47.Kittiwatchana, W., Imsabai, W., Chanprame, S., & Thongket, T. (2021). Effects of drying rates on quality of Thai hot-chili (Capsicum annuum L.) seed after priming. Agriculture and Natural Resources, 55(5), 863-872.
Journal of Plant Production Research
Volume 32, Issue 4
January 2026
Pages 1-22

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