The effects of salicylic acid on induction somatic embryogenesis and plant pigments carrot of root secondary phloem and petiole explant in B5 medium

Salicylic acid play an important role in plants physiological processes. In this research the effect of Salicylic Acid on induction of somatic embryogenesis and plant pigments synthesis in the phloem and petiole explants of carrot in the B5 medium was investigated. We added treatments with 0, 0.5, 1.5, 2 and 3 mM to culture medium by experimental base on the randomized design to investigate the effect of salicylic acid on somatic embryogenesis. Result showed that somatic embryogenesis in 0.5 mM treatment was more than other treatments and the higher concentration (1.5, 2, 3 Mm) reduced somatic embryogenesis. Also the effect of salicylic acid on chlorophyll, Anthocyanin, caratonoid synthesis of explants at realization phasein culture medium was studied. The maximum amount of Caratonoid, Chlorophyll and Anthocyanin was observed at 0.5 mM that was significant between treatments (P<0.01). By more than 0.5 mM concentration, both pigment synthesis and embryogenesis were decreased. Compression among embryogenesis of different carrot explants showed that embryogenesis in petiole explants was more than phloem. Therefore, it could be concluded that probably salycilic acid have different physiological effect at different concentration.