Assessment of Polymorphism in Citrus Genotypes Using RAPD Molecular Markers

It is important to identify the genetic diversity and phylogenic relationships for achieving desirable citrus cultivars. In present investigation RAPD markers were used to determine genetic diversity among 29 citrus genotypes. From 19 used primers, 248 polymorphic bands were amplified. Primers OPA-07 and OPA-05 (with 19 and 8 amplified bands), produced maximum and minimum polymorphic bands, respectively. The average number of polymorphic bands was estimated as 13 for each primer. Similarity among samples was calculated using NTsys software and Jaccard coefficient. Rang of similarity was 0.14-0.97 based on polymorphic bands with average of 0.62. Cluster analysis has been done based on Jaccard's similarity matrix and the UPGMA method. Cluster analysis has divided citrus genotypes into five separate groups. According to the similarity matrix results, the lowest similarity (0.14) was belonged to local mandarin and Pommelo and the highest similarity (0.97) was belonged to unknown natural types G74 and G73. In current research Pommelo and mandarin confirmed as true species of citrus in distinct cluster. Determination of genetic diversity in citrus provides useful information for breeding programs, selection and improving cultivars.