Evaluation of media elements and materials on petiole somatic embryogenesis of Carrot (Daucus carota L.)

Somatic embryogenesis is a new approache to plant mass in vitro propagation. This method influences by elements and their components in medium. In order to investigate the effects of different media and it^,s role on somatic embryogenesis of Carrot, a CBD experiment with four replications (including 12 treatments) was conducted. To do this, Carrot Petioles (with 1 cm dimension) in induction phase were cultured in three different media including MS, B5 and NL. After three weeks, induced organs were transported to realization phase and embryo counting was begun six weeks after realization phase during different embryogenesis stages. Results indicated that studied media had significant differences in respect to somatic embryogenesis (P<0.01). The B5 and MS had the highest and the lowest number of globular, heart, torpedo and seeding, respectively. Results of stepwise regression revealed that the calcium was the most determinant element on induction and embryogenesis evaluation. The Phosphorus and Potassium also were placed in next two priorities. Correlation results show that, Potassium was positively, but Ammonia, Phosphorus and Calcium were negatively correlated with embryo number. Also, results stated that low embryo number in MS media stewed from lacking of casein hydrolysate in media.