The study of QTLs related to seed vigour under stress caused to Sorbitol in rice

In order to genetic analysis of rice seed vigour, a cross was carried out between Tarom Mahalli (tolerant to osmotic stress) and Khazar (sensitive to osmotic stress) crosses. Linkage map based on SSR markers was derived using 74 polymorphic markers and 192 F2 individuals. Germination rate, radicale and plumule length were recorded from 20 plants per 192 F3 families. The loci related to germination traits, were detected using of composite interval mapping. This map covered 1231.5 cM of the genome. qGR-1b on chromosome 1and qRL-12a on chromosome 12 explained 23% and 25% of phenotypic variability related to germination rate and radical length, respectively. The allele from Tarom Mahalli parent in qRL-12a increased root length, 6.70 mm. Major QTLs related to shoot length were detected on chromosomes of 5 and 12. qGR-1b, qRL-12a, qRL-5 and qSL-12 could be used as a potential condidate to be genetically manipulated by marker-assisted selection in rice germination traits breeding methods.