Detection of leaf rust resistance gene Lr32 in Iranian wheat varieties and lines using infection-type data test and molecular markers linked to the Lr32

Puccinia recondite (Leaf rust) is an endemic disease in Iran; this disease can affect the productivity and quality of wheat, that occurring in all wheat growing regions. Genetic resistance to leaf rust of wheat is the most important strategy to control the diseases and 51 leaf rust resistance genes (Lr gene) are reported in wheat to provide resistance to leaf rust. This study aims to identify presence or absence of leaf rust resistance gene Lr32 in 48 Iranian wheat cultivars and lines using of infection-type data test and molecular markers linked to the leaf rust resistance gene. Infection-type analysis based on international method of Browder (1973) with 6 leaf rust pathotype known in Iran, revealed that, first, 17 out of 48 cultivars and lines have effective resistance to race virulence of leaf rust gene Lr32, second, 25 cultivars and lines foresight probability presence resistance gene Lr32 or genes with similar effect, and at last, absence of leaf rust resistance gene Lr32 revealed in 6 cultivars and lines. In molecular part, using known RAPD molecular markers (S49 and S421), evaluated possibility of present of 1100 bp and 640 bp fragments that closely linked to leaf rust resistance gene Lr32 in 48 wheat cultivars and lines. In according with expectation, detected 1100 bp and 640 bp fragments in near-isogonic's Lr gene but none of cultivars, lines and negative control line (Bolani and Agra Local), showed amplification related resistance gene fragments. Result of molecular Markers and infection-type data test showed absence of leaf rust resistance gene Lr32 in 48 Iranian wheat cultivars and lines.