Embryogenesis of a commercial and a native tomato cultivar using different culture media

 
Embryogenesis is an effective method for producing a huge number of genetically modified plants with selective traits. In order to determine suitable nutritional media and organs for embryogenesis with a commercial and a native tomato cultivar, they were tested with three culture media using a factorial completely randomized design with four replications. Pieces of in-vitro cultured hypocotyls and seedling roots were transfered to B5, MS and NL media. Their embryogenesis was investigated when transfered from induction media containing auxin to the free auxin media. No embryogenesis was observed with NL, but it was greater with B5 than MS. Embryogenesis was also significantly greater with native tomato cultivar relative to commercial. one The interactions of cultivarculture media (P<0.01) and cultivarexplants (P<0.05) were both significant. The effect of different sucrose concentrations (10, 20, 30 and 40 g/l) on somatic embryogenesis was investigated with in-vitro culture; 20 g/l showing the best response.