Production of Delphinidin Anthocyanin in the Flower Petals of Gerbera by Agroinfiltration of Flower Color Gene Constructs

Document Type : original paper

Authors

Abstract

Background and objectives:
The study of flower color genes function in gerbera is hampered due to the low efficiency of transformation methods and the long time span needed for production of stably transformed transgenic plants (1). For some functional analysis, the transient expression of genes could be an efficient alternative.
Materials and methods:
Therefore, this study was conducted in two stages. In first stage, the agroinfiltration experiment with 3 flower color constructs (1-pBIH-35S-CcF3´5´H: with one gene, 2-pBIH-35S-Del2: with 3 genes and 3-pBIH-35S-Del8: with 5 genes) in 12 cultivars of gerbera was investigated. Agroinfiltration of gerbera petals were performed by Agrobacterium tumefaciens strain EHA 101 harboring binary vectors pBIH that contained one or more genes of flavonoid 3ʹ 5ʹ-hydroxylase (F3´5´H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), flavanone 3β-hydroxylase (F3H), chalcone isomerase (CHI) and hygromycin phosphotransferase (hpt).
Results:
Visual observations of injected petals showed that cultivars with pink color have shifted flower color from pink to blue and produced delphinidin. In the second stage, this experiment was repeated with 4 pink cultivars (‘Aqua Melone’, ‘Bismarck’, ‘Esmara’ and ‘Rosalin’) and mentioned constructs. The results of HPLC analysis of 4 anthocyanins (delphinidin, cyaniding, pelargonidin and peonidin) showed that the injected petals of ‘Bismarck’ cultivar with pBIH-35S-Del8 construct have the highest delphinidin production.
Conclusion:
Therefore, we can suggest ‘Bismarck’ cultivar of gerbera for stable transformation of genes involving production of anthocyanins for change of flower color particularly production of delphinidin.
Background and objectives:
The study of flower color genes function in gerbera is hampered due to the low efficiency of transformation methods and the long time span needed for production of stably transformed transgenic plants (1). For some functional analysis, the transient expression of genes could be an efficient alternative.
Materials and methods:
Therefore, this study was conducted in two stages. In first stage, the agroinfiltration experiment with 3 flower color constructs (1-pBIH-35S-CcF3´5´H: with one gene, 2-pBIH-35S-Del2: with 3 genes and 3-pBIH-35S-Del8: with 5 genes) in 12 cultivars of gerbera was investigated. Agroinfiltration of gerbera petals were performed by Agrobacterium tumefaciens strain EHA 101 harboring binary vectors pBIH that contained one or more genes of flavonoid 3ʹ 5ʹ-hydroxylase (F3´5´H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), flavanone 3β-hydroxylase (F3H), chalcone isomerase (CHI) and hygromycin phosphotransferase (hpt).
Results:
Visual observations of injected petals showed that cultivars with pink color have shifted flower color from pink to blue and produced delphinidin. In the second stage, this experiment was repeated with 4 pink cultivars (‘Aqua Melone’, ‘Bismarck’, ‘Esmara’ and ‘Rosalin’) and mentioned constructs. The results of HPLC analysis of 4 anthocyanins (delphinidin, cyaniding, pelargonidin and peonidin) showed that the injected petals of ‘Bismarck’ cultivar with pBIH-35S-Del8 construct have the highest delphinidin production.
Conclusion:
Therefore, we can suggest ‘Bismarck’ cultivar of gerbera for stable transformation of genes involving production of anthocyanins for change of flower color particularly production of delphinidin.

Keywords

Main Subjects

References

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Journal of Plant Production Research
Volume 23, Issue 4
February 2017
Pages 145-164

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