Evalution the symbiosis of three mycorrhizae fungi species on biochemical characteristics of Kentucky bluegrass (Poa pratensis) and Agropyron (Agropyron elongatum) turfgrasses under drought stress conditions

Document Type : original paper

Author

Dept. of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan

Abstract

Background and objectives: Turfgrasses are the most important ground covers with high dining leg strength that rarely replacement can be found for them in landscape. Limitation of water resources especially in arid and semi-arid regions is one of the main challenges in turf management. Mycorrhizae symbiosis with plants is one of the ways to reduce the adverse effects of drought stress.

Materials and Methods: In order to investigate the effects of three species of mycorrhizae fungi (G. clarum, G. fasiculatum, G. mosseae and Non fungi) and drought stress (80, 55 and 30 percent of field capacity) on the biochemical characteristics of turfgrass species, Poa pratensis and Agropyron elongatum, a factorial experiment based on randomized complete block design was carried out in the greenhouse of the Faculty of Agricultural Sciences, University of Guilan in 2015. Following the establishment of turfgrass that lasted about 60 days, drought stress was applied. The traits of ascorbate peroxidase (APX), peroxidase (POD) and catalase (CAT) activity and malondialdehyde, proline, total protein of leaves and root colonization percent were studied.

Results: According to the results Agropyron showed the highest root colonization with G. mosseae while Poa showed the lowest colonization with G. clarum. Drought stress reduced the root colonization of Agropyron and poa with G. mosseae but increased the root colonization of them with G. clarum and G. fasiculatum. Drought stress increased the activities of antioxidant enzymes, proline, malondialdehyde and total protein in the leaves of studied grasses. Mycorrhizal fungi, especially under drought stress increase the activity of antioxidant enzymes and proline content, but decreased the malondialdehyde content. There were significant differences between fungi species and grass species in terms of impact on the above mentioned traits. So that Agropyron with G. mosseae but Poa with G. clarum showed the highest leaf APX activity. In the highest level of stress grasses symbiotic with G. mosseae had the highest POD activity while under mild stress condition highest enzyme activity was observed in leaves of grasses symbiotic with G. fasiculatum. Grass response to mycorrhizal fungi species at different levels of drought stress on CAT activity was different. In moderate stress conditions Agropyron symbiotic with G. mosseae had a highest CAT activity, while in the highest level of stress Agropyron symbiotic with G. fasiculatum had a highest enzyme activity but in Poa in both moderate and severe stress the highest enzyme activity was related to grasses that had not been inoculated with mycorrhizae fungi. G. mosseae caused the proline content of grass leaves at the highest level of drought stress more than 130 percent compared to without mycorrhizae situation increased, but malondialdehyde more than 32 percent decreased. In moderate stress this rate was respectively 70 percent increase and 25 percent decrease.

Conclusion: The effect of mycorrhizal fungi species on the activity of antioxidant enzymes in leaves of studied turfgrasses under drought stress was different However, in general, mycorrhiza symbiosis with increases of antioxidant activity and proline could reduce the amount of lipid peroxidation in leaves of studied turfgrasses. In this regards the G. mosseae was more effective.

Keywords

Main Subjects

References

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Journal of Plant Production Research
Volume 24, Issue 3
January 2018
Pages 27-46

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