Evaluation Genetic Diversity of some Tall Chrysanthemum (Chrysanthemum morifolium Ramat.) using Morphological Traits in Khorramabad conditions

Document Type : original paper

Abstract

Background and objectives: In the field of ornamental plants breeding, extensive research has been carried out using morphological, biochemical and molecular markers, but only a few reports have been presented in the field of genetic diversity of Chrysanthemums by morphological and molecular markers. Therefore is important for further identification of germplasm and cultivars of Chrysanthemums in Iran. This study was conducted to evaluate the genetic diversity of 15 cultivars of Chrysanthemums based on morphological characteristics and selection of compatible cultivar with Khorramabad region.

Materials and methods: This experiment was carried out in a randomized complete block design with three replications at the Agricultural Research Station of Lorestan University. quantitative trait such as stem height, petiole length, leaf length, leaf width, number of lateral branches of the plant, leaf to width ratio, bud diameter, the diameter of the disc, flowering date, number of florets tab, branch diameter, number of flowers, leaf length to petiole length ratio, and etc. were measured according to the relevant Descriptor. All characters of the flower measured in the full opening of the first flowers and were used 10 mature leaves in the middle of each plant for evaluation the characteristics leaves.
Result: Based on variance analysis of the data, most the traits examined in cultivars were significant differences (P≤0.01). Results showed that the correlation between the number of flowers with the number of lateral branches, and main branch diameter with the extent of plant there was a significant and positive correlation. The most significant and negative correlation (r = -0.82) was observed between length / width of leaf with leaf area. Based on the results obtained from principal components analysis, the six main and independent components with Eigen values than greater one could justify 82.5 percent of the total variation. The first component, Justified 27.4 percent of the total variation. Cluster analysis cultivars in Euclidean distance of 63.24, were categorized into two main groups. The cultivars "Dila", "Afshan", "Elika" and "Yasamin" in the first group In terms of leaf characteristics have most similar together of leaf traits were most similar. The cultivars "Oran", "Taban3" and "Nadia2" were separated, from the rest cultivars with the highest height branch

Conclusion: Based on the results leaf size, petiole length, branches length, number of branches and number of flowers in plant traits were most important to recognize the diversity of cultivars of Chrysanthemum. With using some cultivars belonging to the first cluster with large flowers and cultivars in the second cluster with a height of more branches as a parent can be began a new cycle of Chrysanthemum breeding program.

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  1. Acquaah, G. 2007. Principles of Plant Genetics and Breeding. Blackwell Publishing Ltd, 569.
  2. Darabi, F., Ehteshamnia, A., Nazarian-Firouzabadi, F., Roien, Z. and Shafie, M.R. 2016. Evaluation of Genetic Diversity among some of Chrysanthemum cultivars using Morphological and SSR molecular markers. Lorestan University. Khorramabad. Iran. 92 p. (In Persian)
  3. Farshadfar, E. 1998. Application of quantitative genetics in plant breeding. Razi University Press. Kermanshah, Iran. 537 p.
  4. Ghahsareh, M. and Kafi, M. 2009. Floriculture. Author Publishing, Esfahan, Iran, 313 p. (In Persian)
  5. Johnson, R.A. and Wichern, D.W. 1982. Applied Multivariate Statistical Analysis. Prentice Hall International Inc. New York.
  6. Khadari, B., Breton, C., and Moutier, N. 2003. The use of molecular markers for germplasm management in a French olive collection. Theor. Appl. Gen. 106: 521-529.
  7. Kiamohammadi, F., Abdusi, V., Moradi, P., Shafiee, M.R. and Arab, S. 2012. Evaluation of Genetic Diversity among Some of Chrysanthemum (Chrysanthemum morifolium Ramat.) cultivars using morphological characteristics. J. Agri. Plant Breed. 8(4): 35-43. (In Persian)
  8. Kim, I.S., Koppula, S., Park, P.J., Kim, E.H., Kim, C.G., Choi, W.S., Lee, K.H. and Choi, D.K. 2009. Chrysanthemum morifolium Ramat. (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-inducedcytotoxicity. J. Ethnopharmacol. 126: 447-454.
  9. Krichen, L., Audergon, J.M. and Trifi-Farah, N. 2012. Relative efficiency of morphological characters and molecular markers in the establishment of an apricot core collection. Heredity. 149: 163-172.

10. Langton, F.A. 1989. Inheritance in Chrysanthemum morifolium Ramat. Heredity.62: 419-423.

11. Lin, L.Z. and Harnly, J.M. 2010. Identification of the phenolic components of Chrysanthemum flower (Chrysanthemum morifolium Ramat). Food Chem.120: 319-326.

12. Nvfrsty, M. 2007. Statistics in Economics and Business. Rasa. Tehran. (In Persian)

13. Pearson, K. 1901. On lines and planes of closest fit to systems of points in space. Philosophical.

14. Roein, Z. 2013. Study of morphological and biochemical characteristics among Some of Chrysanthemum (Chrysanthemum morifolium) and association analysis of their relationship with molecular markers, Ph.D. Thesis. Faculty of Agriculture, University of Guilan 17. (In Persian)

15. Roein, Z., Hassanpour Asil, M. and Sabouri, A. 2015. Study morphological traits, genetic evaluation and grouping Chrysanthemum cultivars. J. Prod. Proc. Crop Agri. and Garden. 16: 345- 359.

16. Shao, Q.S., Guo, Q.S., Deng, Y.M. and Guo, H.P. 2010. A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers. Biochem. Syst. Ecol. 38: 1160-1169 p.

17. Shen, W.Q., Sun, H.Y., Wang, Q.M. and Ma, S.L. 2006. Advances in studies on bioactive constituents and pharmacological activities of Chrysanthemum morifolium Ramat. J. Tea. 32: 141-144.

18. Teixeira DaSilva, J.A. 2004. Ornamental Chrysanthemums: improvement by biotechnology. Plant Cell Tissue Organ Culture. 79: 1-18.

19. Wolff, K. and Peters-van Rijn, J. 1993. Rapid detection of genetic variability in Chrysanthemum (Dendranthema grandiflora Tzvelev.) using random primers. Heredity-London, 71: 335-335.

20. www.floraholland.com

21. Zhang, F., Chen, S., Chen, F., Fang, W. and Li, F. 2010. A preliminary genetic linkage map of Chrysanthemum (Chrysanthemum morifolium) cultivars using RAPD, ISSR and AFLP markers. Sci. Hort. 125: 422-428.