Effect of polyamines on antioxidative responses of safflower (Carthamus tinctorius) under drought stress

Document Type : original paper

Authors

1 PhD student of Plant Physiology Department of Plant Science Faculty of Natural Science University of Tabriz

2 Associated professor, Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz

3 Professor. Department of Plant Eco-physiology, Faculty of Agriculture, University of Tabriz

4 Assistant professor, Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz

Abstract

Background and objectives: Drought stress is the most common abiotic factor which reduces the plant growth and development more than other factors. Thus, identification of the effective factors to increase drought tolerance of plants is necessary. The plants increase antioxidant compounds to overcome the oxidative stress. Polyamines as growth regulators play important roles in maintaining cell membrane stability and reducing ROS generation under drought stress. Carthamus tinctorius is an industrial, medicinal and oil crop from Asteraceae family. Many studies on other plants showed that polyamines increase plants tolerance to environmental stresses, but response of safflower to different concentrations of putrscine+spermine under drought stress is not clear. Therefore, this research was conducted to investigate the effect of these polyamines on enzymatic and non-enzymatic antioxidants activities, lipid peroxidation and membrane stability of safflower.
Materials and methods: This experiment was carried out as a factorial arrangement based on randomized complete block design with three replications in a greenhouse at the University of Tabriz in 2016-2017. In this research, the effect of different putrescine+spermine concentrations (0 + 0, 40 + 40, 40 + 60, and 60 + 40 µM) on the activities of antioxidant enzymes (APX, CAT, SOD and POX), MDA and H2O2 contents and electrolyte leakage, non-enzyme antioxidants (phenols, flavonoids and anthocyanins) in safflower were studied under well-watering (100% field capacity) and limited-watering (40% field capacity). The plants were harvested at 6-7 leaves stage for different measurements.

Results: Irrigation and foliar application of polyamines had significant effects on enzymatic and non-enzymatic antioxidants, MDA, H2O2 contents and electrolyte leakage. Interaction of these factors was significant for all traits. Activities of enzymes such as CAT, POX and SOD, total flavonoid of leaves and anthocyanins of shoots were significantly increased in stressed than in non-stressed plants. Moreover, spray of 40+60 µM and 60+40 µM putrescine+spermine, significantly enhanced the activity of CAT and SOD and anthocyanins contents in stressed-plants. Drought stress increased malondialdehyde, H2O2 and electrolyte leakage in leaves. MDA and electrolyte leakage significantly diminished as a result of putrescine+spermine application.

Conclusion: Water deficit had a negative effect on the growth of safflower via induction of oxidative stress. In general, the application of 40+60 µM and 60+40 µM putrescine+spermine were effective in reducing ROS caused by water deficit. Foliar spray of 60+40 µM putrescine+spermine reduced lipid peroxidation, electrolyte leakage and H2O2 content through increasing antioxidant capacity and flavonoids and anthocyanins, leading to improve drought tolerance of safflower. The results of this study showed that foliar application of 40+60 µM and 60+40 µM putrescine+spermine can be used to mitigate harmful effects of drought stress at early stages of plant growth

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References

1.Amri, E. and Shahsavar, A.R. 2010. Response of lime seedling (Citrus aurantifolia L.) to exogenous spermidine treatments under drought stress. Aust. J. Basic. Appl. Sci. 4: 9. 4483-4489.
2.Ashraf, M. and Ali, Q. 2008. Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environ. Exp. Bot. 63: 1. 266-273.
3.Bitrián, M., Zarza, X., Altabella,T., Antonio, F. and Alcázar, R.2012. Polyamines under Abiotic Stress: Metabolic Crossroads and Hormonal Crosstalks in Plants. Metab. 2: 3. 516-528.
4.Boominathan, R. and Doran, P.M. 2002. Ni- Induced oxidative stress in roots of the Ni hyper accumulator, Alyssum bertolonii. New Phytol. 156: 2. 205-215.
5.Chance, B. and Maehly, A.C. 1955. Assay of catalases and peroxidases: Methods Enzymol. 2: 764-755.
6.Chang, C., Yang, M., Wen, H. andChern, J. 2002. Estimation of total flavonoid content in Propolis by two complementary colorimetric methods.J. Food Drug Anal. 10: 178-182.
7.Daneshmandi, M.Sh. and Azizi, M. 2009. Survey of effect of water stress and application of  mineral zeolite on quantity and quality properties of Ocimum basilicum L. var. keshkeny levelu) In: 6th Congress of Iranian Horticultural Science, Iran, 1-2 March 2009, Pp: 123-129. (In Persian)
8.Farhangi-Abriz, S. and Ghassemi-Golezani, K. 2018. How can salicylic acid and jasmonic acid mitigate salt toxicity in soybean plants? Ecotoxicol. Environ. Saf. 147: 1010-1016.
9.Farooq, M., Wahid, A. and Lee, D.J. 2009. Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiol. Plant. 31: 1. 937-945.
10.Groppa, M.D. and Benavides, M.P. 2008. Polyamines and abiotic stress: recent advances. Amino Acids. 34. 1: 35-45.
11.Hajiboland, R. and Ebrahimi, N. 2011. Growth, photosynthesis and phenolic metabolism in tobacco plants under salinity and application of polyamines. Iran. J. Plant Biol. 3: 1. 13-26. (In Persian)
12.Harinasut, P., Poonsopa, D., Roengmongkol, K. and Charoensataporn, R. 2003. Salinity effects on antioxidant enzymes in mulberry cultivar. Sci. Asian. 29: 109-113.
13.Hojati, M., Modarres-Sanavy, S.M.M., Karimi, M. and Ghanati, F. 2011. Responses of growth and antioxidant systems in Carthamus tinctorius under water deficit stress, Acta Physiol. Plant. 33: 1. 105-112.
14.Hussain, S., Farooq, M., Wahid, M.A. and Wahid, A. 2013. Seed primingwith putrescine the drought resistance
of maize hybrids. Int. J. Agric. Biol.15: 6. 1349-1353.
15.Krouma, A., Fujimura, T. and Abdely, C. 2015. Growth, photosynthetic activity and water relations three Tunsian chickpea genotypes (Cicer arietinum L.) subjected to a progressive water deficit stress. Int. Res. J. 5: 1. 206-214.
16.Lutts, S., Kinet, J.M. and Bouharmont, J. 1996. NaCl- induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot. 78: 389-398.
17.Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkinafasa honey, as well as their radical scavenging activity. Food Chem. 91: 3. 571-577.
18.Mirzaee, M., Moini, A. and Ghanati, F. 2013. Effects of drought stress on the lipid peroxidation and antioxidant Enzyme activities in two canola (Brassica napus L.) cultivars. J. Agric. Sci. Technol. 15: 593-602.
19.Mita, S., Murano, N., Akaike, M.and Nakamura, K. 1997. Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gen for beta-amylase and on the accumulation of anthocyanin that is inducible by sugars. Plant J. 11: 4. 841-851.
20.Mustafavi, S.H., Shekari, F., Nasiri,Y. and Hatami-Maleki, H. 2015. Nutritional and biochemical response of water-stressed valerian plants to foliar application of spermidine. Biol. Forum. Int. J. 7: 1. 1811-1815.
21.Nazarli, H., Hadian, J. and Ahmadi, A. 2015. Evaluation of putrescine effect in drought tolerance inducing and changing of enzyme activities in Matricaria Chamomilla L. plant. Iran. J. Agric. Sci. 46: 2. 222-293.
22.Obinger, C., Maj, M., Nicholls, P. and Loewen, P. 1997. Activity, peroxide compound formation, and heme d synthesis in Escherichia coli HPII catalase. Arch Biochem. Biophys.342: 1. 58-67.
23.Pal, M., Szalai, G. and Janda, T. 2015. Polyamines are important in abiotic stress signaling. Plant Sci. 1: 1. 1-34.
24.Saikat, P. and Aryadeep, R. 2016. Seed priming with spermine ameliorates salinity stress in the germinated seedlings of two rice cultivars differing in their level of salt tolerance. Trop. Plant Res. 3: 3. 616-633.
25.Salehi-lisar, S.Y. and Bakhshayeshan-Agdam, H. 2016. Drought stress tolerance in plants: causes, consequences and tolerance. P 35-50,In: M.A. Hossain, Sh.H. Wani, S. Bhattacharjee, D.J. Burritt and L.S. Phan Tran, Eds. Springer Press. London.
26.Shukla, V., Ma, Y. and Merevitz, V. 2015. Creeping Bentgrass responses to drought stress and polyamine application, J. Am. Soc. Hort. Sci.140: 1. 94-101.
27.Winterbourn, C.C., Mc Grath, B.W.and Carrell, R.W. 1976. Reactions involving superoxide and normal unstable hemoglobins, Biochem. J.155: 3. 493-502.
28.Yamaguchi, K., Takahashi, Y., Berberich, T., Imai, A., Takahashi, T.and Michael, A.J. 2007. A protective role for the polyamine spermine against drought stress in Arabidopsis. Biochem. Biophys. Res. Commun. 352: 2. 486-490.
29.Zamani, Z., Niakan, M. and Gorbanly, M. 2013. Effect of exogenous putrescine in phenolic composition, antioxidant enzymes and nitrate reductase of Hyosyamus niger under drought stress, J. Iran. Plant Ecophysiol. Res. 3: 78-90. (In Persian)
30.Zhang, Zh. and Hung, Zhi. 2013. Effects of endogenous abscisic acid, jasmonic acid, polyamines, and polyamine oxidase activity in tomato seedlings under drought stress. Sci. Hortic.159: 172-177.
31.Zhou, L., Yn, Zh., Danda, P., Xiaojan, W., Yan, P. and Yan, Y. 2015. Polyamine regulates tolerance to water stress in leaves of white clover associated with antioxidant defense and dehydrin genes via involvement in calcium messenger system and hydrogen peroxide signaling. Frontiers Physiol.6: 280. 1-16.
Journal of Plant Production Research
Volume 26, Issue 2
September 2019
Pages 157-171

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