Investigating the optimization of different substrate on some quantitative and qualitative atributies of golden oyster mushroom (Pleurotus citrinopileatus)

Document Type : original paper

Authors

1 horticulture department, agriculture faculty, zabol university

2 Assistant Professor of Horticulture Science (physiology and vegetable breeding), Department of Horticulture and landscaping, Faculty of Agriculture, University of Zabol, Zabol , Iran

3 , Assistant Professor of Horticulture Science, Department of Horticulture and landscape, Faculty of Agriculture, University of Zabol, Zabol , Iran.

4 Staff member of Horticulture Science, Department of Horticulture and landscaping, Faculty of Agriculture, University of Zabol, Zabol , Iran

Abstract

Abstract
Background and objectives:
For the production of various species of oyster mushroom a wide range of lignocellulosic materials different can be used, but to convert biological lignocellulosic waste industrial and agricultural and transport more food from substrate to fruiting body depends to the amount of available foodstuffs in substrate (type culture medium) as well as the power of mycelium degrading agents. Non-localizing technical information on the production of edible mushrooms and the lack of suitable formulation substrate from main problems in the production of commercial and industrial oyster mushroom in the country, so finding a suitable substrate for the production of golden oyster mushroom is first step in the development of this mushroom are be.
Materials and Methods:
In this experiment, various wastes from agricultural and industrial products are included: sawdust, wheat straw, date palm leaf wastes, the combination of sawdust with wheat straw (in equal proportion), combination of sawdust with date palm leaf wastes (in equal proportions), combination wheat straw and date palm leaf wastes (in equivalent ratio) as well as chemical supplements of urea (10g per liter in per kg of substrate based on fresh weight of substrate), manganese sulfate (7μg. kg based on dry matter substrate), ammonium phosphate (15g in liter per kilogram of substrate, based on fresh weight of substrate) and bio additives supplement mycorrhizal (one milliliter of Glomus mosseae inoculum per kg of spawn) and vermicompost (6% fresh weight of substrate). After preparing and adjusting the humidity, the substrate was sterilized with using boiling water. In this experiment, some physiological and biochemical traits, vegetative growth and yield golden oyster mushroom were evaluated.
Results:
The results showed that nitrogen contents of fruit bodies produced on substrate date palm leaf wastes that enrichment with mycorrhizal bio-supplementation were 8.21%, as well as the nitrogen contents of fruit bodies produced on substrate wheat straw that enrichment with ammonium phosphate recorded 4.51%. The completion of the growth stage of mycelium (spawn run) of oyster mushroom in the non-mixed substrate sawdust that enrichment with the mycorrhizal and also in substrate date palm leaf wastes that enrichment with mycorrhiza lasted for 14/60 days, and this stage (spawn run) also took place in substrate wheat straw that supplemented with manganese sulfate lasted 9 days. Also, the highest (2079.00g) and lowest (813.20g) total yield (fresh weight) of fruit bodies were wheat straw substrate that enrichment with manganese sulfate and date palm leaf wastes that enrichment with mycorrhiza respectively.
Conclusion:
In this study, mixed and non-mixed substrates that were enriched were evaluated weight and quality of fruit produced from wheat straw substrate that enrichment with chemical supplement of manganese sulfate (7μg/kg based on dry matter substrate) were statistically superior to other treatments.

Keywords: Food supplements, Organic compounds, Oyster mushrooms, Substrate.

Keywords

Main Subjects


1.Ancona-Mendez, L., Sandoval-Castro, C.A., Casso, R.B. and Capetillo Leal, C.A. 2005. Effect of substrate and harvest on the amino acid profile of oyster mushroom (Pleurotus ostreatus). J. Food Com. Anal. 18: 447-450.
2.AOAC. 1990. Association of Official Analytical Communities. Official Method of Analysis. 18th ed, Washington DC: USA.
3.Asef, M.A. 2016. Iranian Medicinal Fungi. Iranology Publication. 160p.
4.Azizi, U. 1997. Utilization of Agricultural wastes for Production of Oyster Mushroom and Livestock Feed. Agricultural Education Publishing. Karaj. 48p. (In Persian)
5.Bonatti, M., Karnopp, P., Soares, H.M. and Furlan, S.A. 2004. Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. J. Food. Chem .88: 425-428.
6.Chang, S.T. and Miles, P.G. 2004. Mushrooms Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. 477p.
7.Chen, S.Y., Ho, K.J., Hsieh, Y.J., Wang, L.T. and Mau, J.L. 2012. Contents of lovastatin, g-aminobutyric acid and ergothioneine in mushroom fruiting bodies and mycelia. LWT-Food Sci. Technol. 47: 274-278.
8.Cunha-Zied, D. and Pardo-Giménez, A. 2017. Edible and Medicinal Mushrooms: Technology and Applications. 592p.
9.Curvetto, N.R., Figlas, D., Devalis, R. and Delmastro, S. 2002. Growth and productivity of different Pleurotus ostreatus strains on sunflower seed hulls supplemented with N-NH+4 and/or Mn. Bioresour. Technol. 84: 171-176.
10.Dias-Nunes, M., Rodrigues da-Luz, J.M., Albino-Paes, S., Oliveira-Ribeiro, J.J., de Cássia Soares da Silva, M. and Megumi-Kasuya, M.C. 2012. Nitrogen supplementation on the productivity and the chemical composition of oyster mushroom. JFR. 1: 2. 113-119.
11.Elhami, B., Alemzadeh-Ansari, N. and Sedighie-Dehcordie, F. 2008. Effect of Substrate Type, Different Levels of Nitrogen and Manganese on Growth and Development of Oyster Mushroom (Pleurotus florida). Dyn. Biochem. Process Biotech. Mol. Biol. 2: 1. 34-37.
12.Emami, A. 1996. Plant decomposition methods. Agricultural Research, Education and Extension Organization (AREEO). Soil and Water Research Institute.(In Persian)
13.Girmay, Z., Gorems, W., Birhanu, G. and Zewdie, S. 2016. Growth and yield performance of Pleurotus ostreatus (Jacq. Fr.) Kumm (oyster mushroom)on different substrates. AMB Express.6: 1. 87.
14.Gold, M.H., Wariishi, H. and Valli,K. 1989. Extracellular peroxidases involved in lignin degradation by the white-rot basidiomycete Phanerochaete chrysosporium. In: J.R. Whitaker andP.E. Sonnet (eds) 10-Biocatalysis in Agricultural Biotechnology, Vol. ACS Symp. Ser. No. 389, The American Chemical Society, Washington, DC.Pp: 128-140.
15.Hatakka, A. 2001. Biodegradation of lignin. Wiley- VCH, Germany.
16.Hejazi, A., Shahrudi, M. and Ardforush, M. 2007. Index method of plant analiyes (7th ed.). (pp. 197-234.) (In Persian)
17.Hoa, H., Wang, C.L. and Wang, C.H.2015. The effects of different substrateson the growth, yield and nutritional composition of two oystermushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiol. 43: 4. 423-434.
18.Jafarpour, M., Poursaeid, N., Jalali Zand, A., Golparvar, A.R. and Behdad, M. 2009. Effect of some of the wastes of agricultural conversion industries and food supplements on some of the specifications of the edible mushroom (Pleurotus florida). J. Res. Agric. Sci.4: 2. 188-203.
19.Jafarpour, M. and Eghbalsaeed, S. 2012. High protein complementation with high fiber substrates for oyster mushroom cultures. AJB. 11: 14. 3284-3289.
20.Makela, M., Galkin, S., Hatakka, A. and Lundell, T. 2002. Production of organic acids and oxalate decarboxylase in lignin-degrading white rot fungi. Enzyme Microb Technol. 30: 542-549.
21.Mandeel, Q., Al-Laith, A. and Mohamed, S.A. 2005 .Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. WorldJ. Microbiol. Biotechnol. 21: 601-607.
22.Mapanao, K.M., Abella, E.A., Aquino, D.L. and Kalaw Sofronio, P. 2016.Use of effective microorganisms on enhancing the mycelial growth of Pleurotus florida on unsterilized rice straw J. Biol. Eng. Res. Rev. 3: 1. 30-36.
23.Mohammadi Goltapeh, A. and Pourjam, A. 1994. Principles of Edible Mushroom Cultivation.Tarbiat Modares University Press. Tehran. 556p. (In Persian)
24.Mostofi, Y. and Najafi, F. 2005. Laboratory Manual of Analytical Techniques in Horticulture (Translation). Tehran University Press. 85p. (In Persian)
25.Mottaghi, H. 2006. Oyster Mushroom and other Edible Mushroom, Technology and Producing. Andisheh Farda Publications. 328p. (In Persian)
26.Quds-Valid, A. 2010. Planting and Cultivating Edible and MedicinalFungi (Jun-Cao technology). Iranian Agricultural Science Publishing. 217p.
27.Rahman, M.H., Ahmed, K.U., Roy, T.S., Mandal, M.S.H. and AlamL, M.R. 2013. Effect of chemical fertilizer supplements with rice straw on the growth andyield of oyster mushroom (Pleurotus ostreatus). IJAT. 9: 2. 47-51.
28.Ralph, H. and Kurzman, J.r. 1997. Nutrition from mushrooms, understanding and reconciling available data. Mycosci. 38: 247-253.
29.Richard, T. 2002. The scienceand engineering of composting. http//www.cfe.cornell.
30.Royse, D.J. 1996. Specialty mushrooms. Progress in new crops. In: Proceedings of the Third National Symposium; Indianapolis, Indiana, USA. Alexandria: ASHS Press.
31.Salman-Naeem, M., Asif Ali, M., Sajid, A., Sardar, H., Liaqat, R. and Shafiq, M. 2014. Growth and yield performance of oyster mushroom on different substrates. Mycopath. 12: 1. 9-15.
32.Shashirekha, M.N., Rajarathnam, S. and Bano, Z. 2005. Effects of supplementing rice straw growth substrate with cotton seeds on the analytical characteristics of the mushroom, Pleurotus florida (Block and Tsao). J. Food Chem. 92: 255-259.
33.Stajic, M., Persky, L., Hadar, Y., Friesem, D., Duletić-Laušević, S., Wasser, P.and Nevo, E. 2006. Effect of copper
and manganese ions on activities of laccase and peroxidases in three Pleurotus species grown on agricultural wastes. Appl Biochem Biotechnol. 128: 87-96.
34.Tajeddin, B. 1994. The effect of enrichment substrate of Pleurotus sajor-kaju and determination of quantitative and qualitative properties. Master's Degree in Food Industries, Faculty of Agriculture, Tarbiat Modarres University. Tehran. 107p. (In Persian)
35.Vetayasupom, S. 2004. Effective microorganisms for enhancing pleurotus ostreatus (Fr.) Kummer production. J. Biol. Sci. 4: 6. 706-710.
36.Weil, D.A., Beelman, R.B. and Beyer, D.M. 2006. Manganese and other micronutrient additions to improve
yield of Agaricus bisporus. Bioresour. Technol. 97: 1012-1017.
37.Yang, J.H., Tseng, Y.H., Chang, H.L., Lee, Y.L. and Mau, J.L. 2004. Storage stability of monascal adlay. Food Chem. 90: 303-309.
38.Yang, W., Guo, F. and Wan, Z. 2013. Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplementedwith cotton seed hull. Saudi J. Biol. Sci. 20: 333-338.
39.Zheng, J.G., Chen, J.C., Yang, J., Zheng, K.B., Ye, X.F. and Huang, Q.L. 2002. Studies on growing edible fungi on improved straw from a dual use rice cultivar. Agr. Sci. China. 1: 8. 871-877.