Study of total phenolic, total flavonoid content and antioxidant potential in various organs of genus Papaver and Glaucium collected from Iran

Document Type : original paper

Authors

1 Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran

2 Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

Abstract

Background and objectives: Papaver and Glaucium are important genera of the family papaveraceae and commonly used in traditional medicine due to the various alkaloids; however, few studies have been carried out on physiological and phytochemicals of these genuses in Iran. This study was undertaken to determine the total phenolic content, total flavonoid content and antioxidant potential in fruit, root and aerial parts (stem and leaf) of genus Papaver and Glaucium collected from different geographical regions of Iran.
Materials and methods: Eight provinces of Iran (Tehran, Alborz, West Azerbaijan, East Azerbaijan, Ardabil, Lorestan, Hamadan, Chaharmahal and Bakhtiari) as the centers of origin for many species of family papaveraceae were chosen for sampling. After species identification (P. tenuifolium, P. dubium, P. bracteatum, P. orientale, P. arenarium, P. lacerum, G. mathiolifolium, G. pulchrum, G. integrrima, G. elegans, G. pulchrum), extraction of samples was conducted using ultrasonic device. Total phenolic content, total flavonoid content and antioxidant potential of fruit, root and aerial parts (stem and leaf) organs were determined using Folin-Ciocalteu assays, aluminum chloride method and DPPH assay respectively.
Results: The results showed that amounts of total phenolic content, total flavonoid content and antioxidant activity were significantly variable both among species and in different plant organs. The amount of total phenolic was ranging from 23.89 to 77.6 mg GAE/g dry weight plant. Total phenolic content was in its highest value (77.6 mg GAE/g DW) in Ardebil sample (P. bracteatum), whereas the lowest level (23.89 mg GAE/g DW) was found in Saridareh-Germi (P. arenarium). The amount of total flavonoid content was ranging from 1.34 to 5.55 mg Que/g dry weight plant. Total flavonoid content was in its highest value (5.55 mg Que/g DW) in Sin-Sorkhehesar sample (G. mathiolifolium), whereas the lowest level (1.34 mg Que/g DW) was found in Malard-Tehran sample (P. tenuifolium). Furthermore, amount of antioxidant activity was ranging from 20.29 to 87.5%. Antioxidant activity was in its highest value (87.5%) in Hir-Ardebil sample (P. dubium), whereas the lowest level (20.29%) was found in Irdmousa sample (P. dubium).
Conclusion: In terms of total phenol, aerial parts of P. bracteatum species; total flavonoid, aerial parts of G. mathiolifolium species and antioxidant potential, root of P. dubium, were better organs and species. These results showed that different genus of papaveraceae family (especially Papaver genus) are promising sources of natural antioxidants and could supply precious data for screening genotypes with high bioactive contents for producing natural antioxidants and other phytochemical compounds valuable for pharma industries.

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1.Akçam, O.E. 2006. Alkaloid Production in Tissue Cultures of Papaver somniferum L. cv. Office-95. Plant Tissue Cult. Biotech. 16: 1-4.
2.Azadmard Damirchi, S. 2010. Edible oils. Amidi. Press, 475p. (In Persian) 
3.Jafari, A. 2004. Plant anatomy. Jihadof university of Mashhad. Press, 640p.(In Persian) 
4.Simon, J.E., Chedwck, A.F. and Craker, L.E. 2017. Poppy. Available from: URL: http://www.newcrop.hort.pardue.edu.
5.Goldblatt, P. 1974. Biosystematic studies in Papaver section Oxytona. Ann. Missouri Bot. Gard. 61: 264-296.
6.Faribarin, J.W. and Helliwel, K. 1977. Papaver btacteatum Lindley: thebaine content in relation to plant development. J. Pharm. Pharmacol. 29: 65-69.
7.Dif, M.M., Benchiha, H., Mehdadi, Z., Benali-Toumi, F. and Bouterfas, K. 2015. Quantification study of polyphenols in different organs of Papaver rhoeas L. Phytothérapie. 13: 314-319.
8.Rezaei, M., Naghavi, M.R., Hosseinzadeh, A.H. and Abbasi, A. 2016. Measurement of some Benzyl isoquinoline Alkaloids in Different Organs of Persian Poppy during Ontogenetical Stages. Chem. Biodivers.13: 539-543.
9.Dianati-Tilki, G., Mirzaei, A., Rezaei, M.B. and Tabari, M. 2013. Effect of Environmental Factors on Poppy active substances (Papaver bracteatum L.) in Mazandaran Province. EJMP. 1: 1-9.
10.Ounaroon, A., Frick, S. and Kutchan, T.M. 2005. Molecular Genetic Analysis of an OMethyltransferase of the Opium Poppy Papaver Somniferum. Acta Hort. 675p.
11.Ratkin, A.V., Evdokimova, L. and IZhanaeva, T.A. 2003. Study on Degradation of Flavonols in Mutants of PoppyPapaver somniferum L. Biol. Bull. 30: 5. 458-463.
12.Reyes-Carmona, J., Yousef, G.G., Marteniz-Peniche, R.A. and Lila, M.A. 2005. Antioxidant Capacity of Fruit Extracts of Blackberry (Rubus sp.) produced in different climatic regions.J. Food Sci. 70: 497-503.
13.Shrififar, F., Moshafi, M.H. and Mansouri, S.H. 2007. In vitro evalution of antibacterial and antioxidant of the essential oil and methanol extract of endemic Zataria  multiflora Boiss. Food Control. 18: 800-805.
14.Meyers, K.J., Watkins, C.B., Pritts, M.P., and Hai-Liu, R. 2003. Antioxidant and anti-proliferative activities of strawberries. J. Agric. Food Chem.51: 6887-6892.
15.Du, G., Li, M., Ma, F. and Liang, D. 2009. Antioxidant capacity and the relationship with polyphenol and Vitamin C in Actinidia fruits. Food Chem. 113: 557-562.
16.Brand-Williams, W., Cuvelier, M.E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant capacity. JFST. 28: 25-30.
17.Zheng, W. and Wang, S.Y. 2001. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem. 49: 5165-5170.
18.Razali, N., Razab, R., Junit, S. and Abdulaziz, A. 2008. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale L.). Food Chem. 111: 38-44.
19.Vogt, T. 2010. Phenyl propanoid biosyntesis. Mol Plant. 3: 2-20.
20.Takeda, K., Yamaguchi, S.H., Iwata, K., Tsujino, Y., Fujimori, T. and Husain, S.Z. 2001. A malonylated anthocyanin and flavonols in blue Meconopsis flowers. Phytochemistry. 56: 4. 373-6.
21.Salmanian, S., Sadeghi Mahoonak, A., Alami, M. and Ghorbani, M. 2014. Evaluation of Total Phenolic, Flavonoid, Anthocyanin Compounds, Antibacterial and Antioxidant Activity of Hawthorn (Crataegus Elbursensis) Fruit Acetonic Extract. JRUMS. 13: 53-66. (In Persian) 
22.Ghasemi, G., Fattahi, M. and Alirezalu, A. 2017. Evaluation of diversity insome phytochemical characteristics among flower extract of wild-growing populations of Rheum ribes L. in Iran. EJMP. 16: 49-61. (In Persian) 
23.Shameh, S., Hosseini, B. and Alirezalu, A. 2018. Evaluation of distributionand phytochemical diversity of Roses species (Rosa spp.) in Northwest of Iran. JOPPR. 24: 31-45. (In Persian) 
24.Amini, S., Hassani, A., Alirezalu, A. and Maleki, R. 2018. Investigation of genetic diversity among Verbascum species in West Azerbaijan provinceby morphological and phytochemical markers. JOPPR. 24: 123-142.(In Persian) 
25.Gholizadeh, N., Hosseini, B. and Alirezalu, A. 2017. Evaluation of diversity in some phytochemical characteristics in leaf extract among some genotypes of Berberis species in North West of Iran. EJMP. 18: 1-12.(In Persian) 
26.Ebrahimzadeh, M.A., Pourmorad, F. and Bekhradnia, A.R. 2008. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr. J. Biotechnol. 7: 3188-92.
27.Bahri-Sahloul, R., Ammar, S., Grec, S. and Harzallah-Skhiri, F. 2009. Chemical Characterisation of Crataegus Azarolus L. Fruit from 14 Genotypes Found in Tunisia. JHSB. 84: 23-28.
28.Urbonaviciute, A., Jakstas, V., Kornysova, O., Janulis, V. and Maruska, A. 2006. Capillary electrophoretic analysis of flavonoids in single-styled hawthorn (Crataegus monogyna Jacq.) ethanolic extracts. J. Chromatogr. A. 1112: 339-344.
29.Bystrická, J., Vollmannová, A., Margitanová, E. and Čičová, I. 2010. Dynamics of polyphenolics formation in different plant parts and different growth phases of selected buckwheat cultivars. Acta Agric. Slov. 95: 225-229.
30.Orhan, I., Ozcelik, B., Kartal, M., Ozdeveci, B. and Duman, H. 2007. HPLC quantification of vitexine-2-O-rhamnoside and hyperoside in three Crataegus species and their antimicrobial and antiviral activities. Chromatographia. 66: 153-157.
31.Dixon, R.A. and Paiva, N.L.1995. Stress-induced phenylpropanoid metabolism. Plant Cell. 7: 1085-1097.
32.Davise, F.S. and Albrigo, L.G. 1994. Citrus. CAB. International Press, Wallingford, UK, 254p.
33.Hemmati, K.H., Ghasemnejad, A., Mashayekhi, K. and Bashiri Sadr, Z. 2012. Site effect on some important flavonoid compounds of Linden tree (Tilia platifolia L.). JOPPR. 19: 141-148. (In Persian) 
34.Tabatabaei Raisi, A., Khaligi, A., and Kashi, A. 2007. Antioxidant activity and chemical compositions of essential oil of aerial parts of Satureja sahendica Bornm. Pharmaceutical Sci. 3: 1-6.(In Persian) 
35.Zovko-Koncic, M., Kremer, D. and Karlovic, K. 2010. Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat. Food and Chem Toxicol. 48: 2176-21.
36.Zeinali, Z., Hemmati, Kh. and Mazandarani, M. 2013. Aut ecology, ethnopharmacology, phytochemistry and antioxidant activity of ferula gummosa boiss in different regions of razavi khorasan province. EJMP. 4: 11-22.(In Persian) 
37.Khatir Nameni, M. and Mazandarani, M. 2011. Total flavonoids and phenolic different organs of medicinal plant Deadly nights hade (Atropa belladonna L.) in the jungle province Tvskstan. National Conference on Medicinal Plants. 2: 2-7. (In Persian)