Enhancement of phenoilc compounds of olive (Olea europaea L.) leaf with soil application of chemical and organic fertilizers

Document Type : original paper

Authors

1 Assistant Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran,

2 Assistant Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

3 Ph.D., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Background and objectives: Olive tree (Olea europaea L.) leaves are a very promising and unique herbs source which have considerable therapeutic applications. Olive leaves contain high amounts of phenolic compounds such as phenolic acids, flavonoids, phenylpropanoids and secoiridoids. Oleuropein (secoiridoid compound) is considered as the main active phenolic compound in olive leaves. Fewer studies are dedicated to the effects of fertilization on the chemical composition of olive leaves. Therefore, the current study aimed to investigate the associated changes in morphological characteristics, mineral profile and oleuropein and quercetin contents in olive leaves fertilized with chemical and organic fertilizers.
Materials and methods: In order to evaluate the effect of different treatments of chemical and organic fertilizers (humic and fluvic acid) on morphological characteristics, macro- and micronutrient elements as well as phenolic compounds (oleuropin and quercetin) of Zard cultivar, two separate experiments were conducted in a randomized complete block design with three replications in the 2013 crop year. The treatments included application of five levels of NPK (0, 50, 100, 150 and 200 kg ha-1) and six levels of humic acid and fluvic acid (0, 5, 7, 10, 12.5 and 15 liters ha-1).
Results: The results of chemical fertilizers showed that the highest plant height (117.7 cm) and branches number (34 branches) showed in N200P200K200 and N100P100K100 kg ha-1, respectively. The most leaves (60 leaves) were obtained in application of 10 liters per hectare of organic fertilizers. Compared to the control, application of 100 and 200 kg ha-1 NPK showed an increase of 30 and 80% of the oleuropine and quercetin content, respectively. The effect of organic fertilizers was only significant on quercetin content, and its maximum (397.7 μg g-1) was measured in 10 g liters-1 treatment. Effect of chemical fertilizer levels on all measured macro- and micronutrients except potassium was significant and the highest measured values of the elements were obtained at N200P200K200 kg ha-1 level. The effect of organic fertilizers levels on magnesium, manganese, zinc and copper content was also significant.
Conclusion: The application of organic fertilizers such as humic and fluvic acid along with chemical fertilizers can improve the morphological characteristics, provide the nutritional requirements of the plant and increase the phenolic compounds of olive leaves.
Conclusion: The application of organic fertilizers such as humic and fluvic acid along with chemical fertilizers can improve the morphological characteristics, provide the nutritional requirements of the plant and increase the phenolic compounds of olive leaves.

Keywords


1.Abdel Mawgoud, A.M.R., El Greadly, N.H.M., Helmy, Y.I. and Singer, M. 2007. Responses of tomato plants to different rates of humic based fertilizer and NPK fertilization. J Appl. Sci. Res.3: 2. 169-174.
2.Andrewes, P., Busch, J.L.H.C., de Joode, T., Groenewegen, A. and Alexandre, H. 2003. Sensory properties of virgin olive oil polyphenols: identification of deacetoxy-ligstroside aglycon as a key contributor to pungency. J. Agric. Food Chem. 51: 1415-1420.
3.Ben Mimoun, M., Loumi, O., Ghrab, M., Latiri, K. and Hellali, R. 2004. Foliar potassium application on olive tree. IPI Workshop on potassium and fertigation development in WANA.
4.Bilgin, M. and Sahin, S. 2013. Effects of geographical origin and extraction methods on total phenolic yield of olive tree (Olea europaea L.) leaves. J. Taiwan Inst. Chem. Eng. 44: 8-12.
5.Bould, C. 1966. Leaf analysis of deciduous trees. In: Nutrition of Fruit Crops (Ed. N.F. Childers). Horticultural publications. Ruthers University, New Jersey. Pp: 651-684.

6.Casenave de Sanfilippo, E., Argüello, J.A., Abdala, G. and Orioli, G.A. 1990. Content of auxin-, inhibitor- and gibbereilin-like substances in humic acids. Biol. Plantarum. 32: 346-351.

7.Cerdan, M., Sánchez-Sánchez, A., Juarez, M., Sanchez-Andreu, J.J., Jorda, J.D. and Bermudez, D. 2007. Partial replacement of Fe (o,o-EDDHA) by humic substances for Fe nutrition and fruit quality of citrus. J. Plant Nutr. Soil Sci. 170: 474-478.
8.Chapman, H.D. and Pratt, P.F. 1961. Method of analysis for soils, plants and waters. University of California. Division of Agricultural Sciences.
9.Chen, Y. and Aviad, T. 1990. Effect of humic substances on plant growth. In: Humic substances in soil and crop sciences. Soil Sci. Soc. Am. J. Pp: 161-187.
10.Elmer, P. 1982. Biochemistry, BC5, BC7. Analytical methods for atomic absorbtion spectrophotometry. Perkin-Elmer Cooperation, Norwark, CT, 200p.
11.Farajzadeh Memari Tabrizi, A., Yarnia, M., Ahmadzadeh, V. and Farajzadeh Memari Tabrizi, N. 2014. Effectof levels of drought tension and concentrations of potassium humate on two hybrids of hybrid corn 604 and 704. Crop Physiol. J. 7: 25. 105-118. (In Persian)
12.Fathi, M.A., Gabr, M.A. and El Shall, .A. 2010. Effect of humic acid treatments on 'Canino' apricot growth, yield and fruit quality. New York Sci. J. 3: 109-115.
13.Fernandez-Escobar, R., Benlloch, M., Barranco, D., Duefias, A. and Ganan, J.A. 1996. Response of olive trees to folk application of humic substances extracted from leonardite. Sci. Hort.66: 191-200.
14.Fernandez-Escobar, R. 2004a. Fertilizacion. In: El cultivo del olivo (Barranco D., Fernandez-Escobar R. and Rallo L.,
Ed. Mundi-Prensa, Madrid, Spain.Pp: 287-320.
15.Fernandez-Escobar, R., Benlloch, M., Herrera, E. and Garcıa-Novelo, J.M. 2004b. Effect of traditional and slow-release N fertilizers on growth of olive nursery plants and N losses by leaching. Sci. Hort. 101: 39-49.
16.Fernandez-Escobar, R., Beltran, G., Sanchez-Zamora, M.A., Garcia-Novelo, J., Aguilera, M.P. and Uceda, M.2006. Olive oil quality decreases with nitrogen over-fertilization. Sci. Hort.41: 1. 215-219.
17.Furneri, P.M., Marino, A., Saija, A., Uccella, N. and Bisignano, G. 2002. In vitro antimycoplasmal activity of oleuropein. Int. J. Antimicrob. Agents.20: 293-296.
18.Gimenez, C., Diaz, E., Rosado, F., Garcia-Ferrer, A., Sanchez, M., Parra, M.A., Diaz, M. and Pena, P. 2001. Characterization of current management practices with high risk of nitrate contamination in agricultural areas of southern Spain. Acta Hort. 563: 73-80.
19.Golmouhammadi, M. 2005. Effect of harvest management and boron utilization on olive yield and biennial bearing. 4th Congress of Iranian Hort. Sci. Mashhad, Iran. (In Persian)
20.Gonzalez, M., Zarzuelo, A., Gamez, M.J, Utrilla, M.P., Jime´nez, J. and Osuna, I. 1992. Hypoglycemic activity of olive leaf. Planta Med. 58: 513-515.
21.Gonzalez-Garcia, F., Catalina, L.and sarminto, R. 1976. Aspectos bioquimicos de la floracion de olive var Manzanillo en relation con factores nutritionales. lnt. coll. plant nurt, Gent.
22.Hakan, C., Vahap Katkat, A., Bulent Asik, B. and Turan, M.A. 2011. Effect of foliar applied humic acid to dry weight and mineral nutrient uptake of maize under calcareous soil conditions communications. Soil Sci. Plant Anal. 42: 1. 29-38.
23.Haukioja, E., Ossipov, V., Koricheva, J., Honkanen, T., Larsson, S. and Lempa, K. 1998. Biosynthetic origin of carbon-based secondary compounds: cause of variable responses of woody plants to fertilization? Chem. 8: 133-139.
24.Hayes, J.E., Allen, P., Brunton, N., Ogrady, M.N. and Kerry, J.P. 2010. Phenolic composition and in vitro antioxidant capacity of four commercial phytochemical products: Olive leaf extract (Olea europaea L.), lutein, sesamol and ellagicacid. Food Chem. Pp: 948-955.
25.IRMO. 2012. Islamic, Republic of Iran Meterological Organization Climatology Methodes, http/www.irmo.ir.
26.Jasmi, K.H., Karduny, F. and Behdani, M. 2009. Evaluation of the use of fertilizers and its impact on the performance of farms. Congress s challenges fertilizer, fertilizer half a century. Tehran Olympic Hotel. 10-12 March.
27.Jemai, H., Bouaziz, M., Fki, I., El-Feki, A. and Sayadi, S. 2008. Hypolipidimic and antioxidant activities of oleuropein and its hydrolysis derivative-rich extracts from Chemlali olive leaves. Chem-Biol Interact. 176: 88-98.
28.Jones, C.G. and Hartley, S.E. 1999. A protein competition model of phenolic allocation. Oikos 86: 27-44.
29.Kainulainen, P., Utriainen, J., Holopainen, J.K., Oksanen, J.A.R.I. and Holopainen, T. 2000. Influence of elevated ozone and limited N availability on conifer seedlings in an open-air fumigation system: effects on growth nutrient content mycorrhiza needle ultrastructure starch and secondary compounds. Glob Change Biol. 6: 345-355.
30.Kaya, M., Atak, M., Khawar, K.M., Ciftci, C.Y. and Ozcan, S. 2005. Effect of presowing seed treatment with Zinc and foliar spray of humic acids on yield of common bean (Phaseolus vulgaris L.). Inter. J. Agric. Biol. 6: 875-878.
31.Khatamian, N., Nabavi Kalat, M. and Bakhsh Kalarestaghi, K. 2011. Effects of humic acid on morphological characteristics and grain yield of triticale cv. Javanilu. The first national conference on new issues in agriculture, Islamic Azad University, Sabzevar Branch. (In Persian)
32.Khayyal, M.T., El-Ghazaly, M.A., Abdallah, D.M., Nassar, N.N., Okpanyi, S.N. and Kreuter, M.H. 2002. Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats. Arznem Forsch. 52: 797-802.
33.Kiritsakis, K., Kontominas, M.G., Kontogiorgis, C., Hadjipavlou-Litina, D., Moustakas, A. and Kiritsakis, A. 2010. Composition and antioxidant activity of olive leaf extracts from greek olive cultivars. J. Am. Oil Chem. Soc.87: 369-376.
34.Le Floch, F., Tena, M.T., Rıos, A. and Yalcarcel, M. 1998. Supercritical fluid extraction of phenol compounds from olive leaves. Talanta. 46: 1123-1130.
35.Lobartini, J.C., Tan, K.H. and Pape,C. 1998. Dissolution of aluminum and iron phosphate byhumic acids. v. 29 (5/6) Commun. Soil Sci. Plant Anal. Pp: 535-544.
36.Lopez-Granados, F., Jurado-Exposito, M., Alamo, S. and Garcia-Torres, L. 2004. Leaf nutrient spatial variability and site-specific fertilization maps within olive (Olea europaea L.) orchards. Eur. J. Agron. 21: 209-222.
 37.Lo Scalzo, R., Scarpati, M.L., Verzengnassi, B. and Vita, G. 1994. Olea europaea chemical repellent to Dacus oleae females. J. Chem. Ecol.20: 1813-1823.
38.Mackowiak, C.L., Grossl, P.R.and Bugbee, B.G. 2001. Beneficial effects of humic acid on micronutrient availability to wheat. Soil Sci. Soc. Am. J. 65: 1750-1744.
39.Malakouti, M.J. and Tabatabaei, S.J. 2001. Innovative approach to balanced nutrition of fruit trees. Agric. Educ. Publ., Tehran. 191-200. (In Persian)
40.Malik, N.S.A. and Bradford, J.M. 2006. Changes in oleuropein levels during differentiation and development of floral buds in ‘Arbequina’ olives. Sci. Hort. 110: 274-278.
41.Mato, M.C., Olmedo, M.G. and Mendez, I. 1972. Inhibition of indoleacetic acid oxidase by soil humic acids fractionated in Sephadex. Soil Biol. Biochem.4: 469-473.
42.Mayhew, L. 2004. Humic acid substances in biological agriculture. Eco. Agric. 34: 82.
43.Mohammadipour, E., Golchin, A., Mohammadi, J., Negahdar, N. and Zarchini, M. 2012. Effect of humic acid on yield and quality of marigold (Calendula officinalis L.). Ann. Biol. Res. 3: 5095-5098.
44.Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. 2002. Physiological effects of humic substances on higher plants. Soil Biol. Biochem. 34: 1527-1536.
45.Nasoti Miandoab, R., Samawat, S. and Tehrani, M.M. 2010. Properties of humic acid fertilizer on plant and soil. Agric. Food. 101: 55-53.
46.Natesan, R., Kandasamy, S., Thiyageshwari, S. and Boopathy, P.M. 2007. Influence of lignite humic acid on the micronutrient availability and yield of blackgram in an alfisol. Sci. World J. 7: 1198-1206.
47.Noori, O., Taheri, M., Tokasi, M. and Gholiyan, A. 2015. Evaluation of tarom olive orchards nutritional status using the deviation from optimum percentage method (DOP). J. Soil Manage. Sust. Prod. 5: 1. 79-95. (In Persian)
48.Omar, S.H. 2010. Oleuropein in olive and its pharmacological effects. Sci. Pharm. 78: 154-133.
49.Ortega-Garcıa, F. and Peragon, J.2009. The response of phenylalanine ammonia-lyase polyphenol oxidase and phenols to cold stress in the olive tree (Olea europaea L. cv Picual). J. Sci. Food Agric. 89: 1565-1573.
50.Owen, R.W., Giacosa, A., Hull, W.E., Haubner, R., Spiegelhalder, B. and Bartsch, H. 2000. The antioxidant/ anticancer potential of phenolic compounds isolated from olive oil. Eur. J. Cancer. 36: 1235-1247.
51.Ozdamar Unlu, H., Unlu, H., Karakurt, Y. and Padem, H. 2011. Changes in fruit yield and quality in response to foliar and soil humic acid application in cucumber. Sci. Res. Essays. 6: 2800-2803.
52.Petridis, A., Therios, I., Samouris, G. and Tananaki, C. 2012. Salinity-induced changes in phenolic compounds in leaves and roots of four olive cultivars (Olea europaea L.) and their relationship to antioxidant activity. Environ. Exp. Bot. 79: 37-43.
53.Petti, S. and Scully, C. 2009. Polyphenols, oral health and disease:A review. J. Dent. 37: 413-423.
54.Puspita Sari, A., Triadiati, T. and Ratnadewi, D. 2017. Effects of shading and fertilizer on the growth and antioxidant content of olives (Olea europaea L.). Pertanika J. Trop. Agric. Sci. 40: 2. 269-278.
55.Rahii, A., Davvodi Fard, M., Azizi, F. and Habiby, D. 2012. Effects of different amounts of humic aacid and response curves in the Dactylis glomerata. Agric. Plant Breed. J.8: 3. 15-28.
56.Roozbahani, A., Ghorbani, S., Mirzaii, M.M. and Ouroj Nia, S. 2013. The effect of soil application of humic acid and fluvic acid on yield and yield component of barley (Hurdeum vulgare L.). J. Agron Plant Breed. 9: 2. 25-33.(In Persian)
57.Rufat, J., Villar, J.M., Pascual, M., Falguera, V. and Arbones, A. 2014. Productive and vegetative response to different irrigation and fertilization strategies of an Arbequina olive orchard grown under super-intensive conditions. Agric Water Manag. 144: 33-41.
58.Samavat, S. and Malakouti, M.2005. The necessity of using organic acids to increase the quantity and quality of agricultural products. Tech. J.,No. 463. Sana publications, Tehran, Iran. (In Persian)
59.Sanchez Sanchez, A., Sanchez Anderu, J., Juarez, M., Jorda, J. and Bermudez, D. 2002. Humic substances and amino acid improve effectiveness of Chelate FeEDDHA in lemons trees. J. Plant Nutr. 25: 11. 2433-2442.
60.SAS Institute. 2002. SAS/STAT user’s Guide, Release G. 12. SAS Institute Cary. North Carolina. USA.
61.Shehata, S.A., Gharib, A.A., El-Mogy, M.M., Abdel-Gawad, K.F. and Shalaby, E.A. 2011. Influence of compost, amino and humic acids on the growth, yield and chemical parameters of strawberries. J. Med. Plant Res. 5: 2304-2308.
62.Sidari, M., Ronzello, G., Vecchio, G. and Muscolo, A. 2008. Influenceof slope aspects on soil chemicaland biochemical properties in a Pinus laricio forest ecosystem of Aspromonte (Southern Italy). Eur. J. Soil Biol.44: 364-372.
63.Soyergin, S., Moltay, I., Genc, C., Fidan, A.E. and Sutcu, A.R. 2002. Nutrient status of olives grown in the Marmora region. International Society for Horticultural Science (ISHS) Acta Hort. 586: 381-383.
64.Taheri, M. and Malakouti, M. 2000. Necessity of optimization use of fertilizers to increase the yield and quality of the olives. Tech. Publ. No. 66. Karaj, Iran. (In Persian)
65.Talhaoui, N., Taamalli, A., Mara Gomez-Caravaca, A., Fernandez-Gutierrez, A. and Segura-Carretero,A. 2015. Phenolic compounds inolive leaves: Analytical determination, biotic and abiotic influence, andhealth benefits. Food Res. Int. Pp: 1-64.
 66.Tartoura, K.A. 2010. Alleviation of oxidative-stress induced by drought through application of compost in wheat (Triticum aestivum L.) plants. Am. Eurasian J. Agric. Environ. Sci.9: 2. 208-216.
67.Tekaya, M., El-Gharbi, S., Mechri, B., Chehab, H., Bchir, A., Chraief, I., Ayachi, M., Boujnah, D., Attia, F. and Hammami, M. 2016. Improving performance of olive trees by the enhancement of key physiological parameters of olive leaves in response to foliar fertilization. Acta Physiol. Plant. 38: 101. 1-12.
68.Tombesi, A., Michalakis, N. and Pastor, M. 1996. Recommendation of the working group on olive farming production techniques and productivity. Olivae. 63: 38-51.
69.Tripoli, E., Giammanco, M., Tabacchi, G., Di Majo, D., Giammanco, S. andLa Guardia, M. 2005. The phenolic compounds of olive oil: structure, biological activity and beneficial effects on human health. Nutr. Res. Rev.18: 98-112.
70.Turan, M.A., Asik, B.B., Katkat, A.V. and Celik, H. 2011. The effects ofsoil-applied humic substances to thedry weight and mineral nutrient uptakeof maize plants under soil salinity conditions. Not Bot Horti Agrobot Cluj Napoca. 39: 171-177.
71.Uccella, N. 2001. Olive biophenols: biomolecular characterization, distribution and phytolexin histochemical localization in the drupes. Trends Food Sci. Technol. 11: 315-327.
72.Vaughan, D. and Linehan, D. 1976. The growth of wheat plants in humic acid solutions under axenic conditions. Plant Soil. 44: 445-449.
73.Visioli, F. and Galli, C. 2001. Antiatherogenic components of olive oil. Curr. Atheroscler Rep. 3: 64-67.74.Visioli, F., Poli, A. and Galli, C.2002. Antioxidant and other biological activities of phenols from olives and olive oil. Med. Res. Rev. 22: 65-75.
 75.Wahing, I.W., Van Houba, V.J.G. and Van der lee, J.J. 1989. Soil and plant analysis, a series of syllabi. Part 7,plant analysis procedure. Wageningen Agriculture University.
76.Zarzuelo, A., Duarte, J., Jimenez,M. and Utrilla, P. 1991. Vasodilator effect of olive leaf. Planta Med.57: 417-419.