Effects of 6-Benzyladenine on total nonstructural carbohydrates and water-soluble carbohydrates of tall fescue leaves subjected to drought stress

Document Type : scientific research article

Authors

1 M.Sc. Student, Dept. of Horticultural Science and Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), Gorgan, Iran

2 Assistant Prof., Dept. of Horticultural Science and Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), Gorgan, Iran,

3 Laboratory Expert, Dept. of Horticultural Science and Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), Gorgan, Iran

Abstract

Abstract
Background and objectives: The effects of external application of cytokinins on turfgrass leaves and their effects on mitigation of drought stress have been reported in a few studies. However, the effect of benzyl adenine on the content of non-total structural carbohydrates and water-soluble carbohydrates of tall fescue leaves ( Festuca arundinacea Scherb.) has never been investigated under different drought stresses regime, which is our goals in this experiment.
Material and methods: A one-month study on tall fescue that was clonally propagated and established in greenhouse laid out based on completely randomized design with at least three replications including four levels of irrigation treatments (100%, 75%, 50% and 25% of field capacity). In addition to the aforesaid treatments, pots with the same irrigation treatments, foliar sprayed by 50 µM of 6-benzyladenine every 7 days. For comparison, in drought stressed control plants, foliar application of 6-benzyladenine did not applied.
Results: Plants grown on 25% of field capacity was surprisingly influenced by foliar application of benzyl adenine as such noticeable return of leaves to normal growth condition was seen. Looking into water-soluble carbohydrates showed a many fold increase compared to control plants. This water-soluble carbohydrates increase at 50% and 25% of field capacity is much more noticeable than the other treatments. Although foliar treatment with benzyl adenine did not reduce the increase in soluble sugars production to the level of control plants, but BA-influenced WSCs content reduced by half in 50% and 25% of field capacity treatment. Drought stress has reduced chlorophyll content and leaf Starch. This chlorophyll reduction at 25% of FC, to great extent compensated with 50 50 µM of 6-benzyladenine . The activity of α-amylase and β-amylase augmented upon drought stress treatment in 75% of FC followed by reduction in 50% and 25% of FC.
Conclusion: A drastic reduction in soil water content and then plant water can activate starch-degrading enzymes, leading to an increase in water-soluble carbohydrates which could either maintain leaf cell homeostasis or produce more ATP to help with drought stress tolerance. A part of starch degradation in leaves can be explained by α-amylase and β-amylase activity. The research result indicated that application of 50 μM benzyl adenine significantly reduced the stress intensity applied to the plant. This treatment, to great extent improved chlorophyll content and photosynthesis at 25% of FC and reduced further starch degradation and its conversion to fructose, glucose and sucrose, thereby preserving the plant's carbohydrate resources.
Key words: Cytokinin, Drought stress, Tall fescue, Water soluble carbohydrates

Keywords

References

1.Adamipour, N., Salehi, H. and Khosh-Khui, M. 2017. Investigation of Morphological and Physiological Indices in Bermudagrass (Cynodon dactylon [L.] Pers.), Tall Fescue (Festuca arundinacea Schreb.) and Their Seed Mixtures under Drought Stress. Iranian J. Hort. Sci. Tech.18: 1. 15-32.
2.Beard, J.B. 1973. Cool season turfgrasses. Turfgrass: Science and Culture. Englewood Cliffs, NJ: Prentice-Hall, Pp: 54-131.
3.Bernfeld, P. 1955. Methods in enzymology
4.Bledsoe, S.W., Henry, C., Griffiths, C.A., Paul, M.J., Feil, R., Lunn, J.E. and Lagrimini, L.M. 2017. The role of Tre6P and SnRK1 in maize early kernel development and events leading tostress-induced kernel abortion. BMC Plant Biol. 17: 1. 74.
5.Blum, A. 2011. Plant breeding forwater-limited environments. Plant Breed. Water Limited Environ. Pp: 1-255.
6.Carrow, R.N. 1996. Drought avoidance characteristics of diverse tall fescue cultivars. Crop Sci. J. 36: 2. 371-377.
7.Chaves, M.M. 1991. Effects of water deficits on carbon assimilation. J. Exp. Bot. 42: 1. 1-16.
8.Chen, Z., Wang, Z., Yang Y., Li, M. and Xu, B. 2018. Abscisic acid and brassinolide combined application synergistically enhances drought tolerance and photosynthesis of tall fescue under water stress. Sci. Hort. 228: 2018. 1-9.
9.Cougnon, M., Baert, J., Van Waes, C. and Reheul, D. 2014. Performance and quality of tall fescue (Festuca arundinacea Schreb.) and perennial ryegrass (Lolium perenne L.) and mixtures of both species grown with or without white clover (Trifolium repens L.) under cutting management. Grass and For. Sci. J.69: 4. 666-677.
10.Cross, J.W., Bonos, S.A., Huang, B. and Meyer, W.A. 2013. Evaluation of heat and drought as components of summer stress on tall fescue genotypes. Hort. Sci. 48: 1562-1567.
12.DaCosta, M. and Huang, B. 2006. Osmotic adjustment associated with variation in bentgrass tolerance to drought stress. J. Amer. Soc. Hort. Sci. 131: 3. 338-344.
13.Dong, S. and Beckles, D.M. 2019. Dynamic changes in the starch-sugar interconversion within plant source and sink tissues promote a better abiotic stress response. J. Plant Phys. 234: 80-93
15.Fry, J. and Huang, B. 2004. Advanced Turfgrass Science and Physiology. John Wileys and Sons, New Jersey.
16.Fu, J. and Huang, B. 2001. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. J. Exp. Bot. 45: 105-114.
17.Fu, J., Huang, B. and Fry, J.D. 2010. Osmotic potential, sucrose level, and activity of sucrose metabolic enzymes in tall fescue in response to deficit irrigation. J. Amer. Soc. Hort. Sci.135: 506-510.
18.Fulton, D.C., Stettler, M., Mettler, T., Vaughan, C.K., Li, J., Francisco, P., Gil, M., Reinhold, H., Eicke, S., Messerli, G., Dorken, G., Halliday, K., Smith, A.M. and Zeeman, S.C. 2008. Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active β-amylases in Arabidopsis chloroplasts. Plant Cell.20: 1040-1058.
19.Ghanem, M.E., Albacete, A., Martínez-Andújar, C., Acosta, M., Romero-Aranda, R., Dodd, I.C. and Pérez-Alfocea, F. 2008. Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.).J. Exp. Bot. 59: 11. 3039-3050.
20.González, J.A., Gallardo, M., Hilal, M., Rosa, M. and Prado, F.E. 2009. Physiological responses of quinoa (Chenopodium quinoa Willd.) to drought and waterlogging stresses: dry matter partitioning. Bot. Stud. 50: 35-42.
21.Griffiths, C.A. and Paul, M.J. 2017. Targeting carbon for crop yield and drought resilience. J. Sci. Food Agric. 97: 14. 4663-4671.
22.Handel, E.V. 1968. Direct micro determination of sucrose. Anal. Biochem. 22: 280-283.
23.Hannaway, D., Fransen, S., Cropper, J., Teel, M., Chaney, M., Griggs, T., Halse, R., Hart, J., Cheeke, P., Hansen, D., Klinger, R. and Lane, W. 1999. Tall Fescue (Festuca arundinacea Schreb.). PNW. 504. Org. State Univ. Ext.
24.Hiscox, J.D. and Israelstam, G.F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57: 1332-1334.
25.Huang, B. and Gao, H. 1999. Physiological responses of diverse tall fescue cultivars to drought stress. Hort. Sci. J. 34: 897-901.
26.Huang, B., DaCosta, M. and Jiang, Y. 2014. Research advances in mechanisms of turfgrass tolerance to abiotic stresses: from physiology to molecular biology. Crit. Rev. Plant Sci. 33: 2-3. 141-189.
27.Huber, S.C. and Huber, J.L. 1996. Role and regulation of sucrose-phosphate synthase in higher plants. Ann. Rev. Plant Biol. 47: 431-444.
28.Jiang, Y. and Huang, B. 2001. Osmotic adjustment and root growth associated with drought preconditioning-enhanced heat tolerance in Kentucky bluegrass. Crop Sci. 41: 1168-1173.
29.Koźmińska, A., Al Hassan, M., Wiszniewska, A., Hanus-Fajerska, E., Boscaiu, M. and Vicente, O. 2019. Responses of succulents to drought: comparative analysis of four Sedum (Crassulaceae) species. Sci. Hort.243: 235-242.
30.Lewandowski, I., Scurlockb, J.M.O., Lindvallc, E. and Christou, M. 2003. The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe. Biom. Bioen. 25: 335-361.
32.Luquet, D., Clement-Vidal, A., Fabre, D., This, D., Sonderegger, N. and Dingkuhn, M. 2008. Orchestration of transpiration, growth and carbohydrate dynamics in rice during a dry-down cycle. Func. Plant Biol. 35: 8. 689-704.
33.McCready, R.M., Guggolz, J.,Silviera, V. and Owens, H.S. 1950. Determination of starch and amylose in vegetables. Anal. Chem. 22: 1156-1158.
34.Merewitz, E., Gianfagna, T. and Huang B. 2011. Photosynthesis, water use and root viability under water stress as affected by expression of SAG12-ipt controlling cytokinin synthesis in Agrostis stolonifera. J. Exp. Bot. 62: 383-395.
35.Merewitz, E.B., Du, H., Yu, W., Liu, Y., Gianfagna, T. and Huang, B. 2011. Elevated cytokinin content in ipt transgenic creeping bentgrass promotes drought tolerance through regulating metabolite accumulation. J. Exp. Bot. 63: 3. 1315-1328.
36.Merewitz, E.B., Du, H., Yu, W., Liu, Y., Gianfagna, T. and Huang, B. 2011. Elevated cytokinin content in ipt transgenic creeping bentgrass promotes drought tolerance through regulating metabolite accumulation. J. Exp. Bot. 63: 1315-1328.
37.Merewitz, E., Gianfagna, T. and Huang, B. 2010. Effects of SAG12-ipt and HSP18.2-ipt. expression on cytokinin production, root growth and leaf senescence in creeping bentgrass exposed to drought stress. J. Am. Soc. Hort. Sci. 135: 230-239.
38.Miller, C.O., Skoog, F., Von Saltza, M.H. and Strong, F.M. 1955. Kinetin,a cell division factor from deoxyribonucleic acid. J. Am. Chem. Soc. 77: 5. 1392-1392.
39.Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426-428.
40.Movahedi, N.S.A.R. and Rezaei, M. 2008. Soil physics. Gorgan University of Agricultural Sciences and Natural Resources. Press, 474p. (In Persian) 
41.Ojeda, J.J., Caviglia, O.P., Agnusdei, M.G. and Eriksen, G.E. 2015. Comparative analysis of water productivity between “Tall fescue” (Festuca arundinacea Schreb.) and “Oats” (Avena sativa L.). In 38th Congreso Argentino de Prod. Anim.
J. 15: 158-158.
42.Omokolo, N.D., Tsala, N.G. and Djocgoue, P.F. 1996. Changes in carbohydrate, amino acid and phenol content in cocoa pods
from three clones after infection with Phytophthora megakarya Bra. Grif. Ann. Bot. 77: 153-158.
43.Paul, M.J., Oszvald, M., Jesus, C., Rajulu, C. and Griffiths, C.A. 2017. Increasing crop yield and resilience with trehalose 6-phosphate: targeting afeast-famine mechanism in cereals for better source-sink optimization. J. Exp. Bot. 68: 16. 4455-4462.
44.Per, T.S., Khan, N.A., Reddy, P.S., Masood, A., Hasanuzzaman, M., Khan, M.I.R. and Anjum, N.A. 2017. Approaches in modulating proline metabolism in plants for salt and drought stress tolerance: Phytohormones, mineral nutrients and transgenics. Plant Physiol. Biochem. 115: 126-140.
45.Prasch, C.M., Ott, K.V., Bauer, H., Ache, P., Hedrich, R. and Sonnewald, U. 2015. Beta-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells. J. Exp. Bot. 66: 19. 6059-6067.
46.Qian, Y.L., Fry, J.D. and Upham, W.S. 1997. Rooting and drought avoidance of warm-season turfgrasses and tall fescue in Kansas. Crop Sci. 37: 905-910.
47.Rabbani, G. and Choi, I. 2018. Roles of osmolytes in protein folding and aggregation in cells and their biotechnological applications. Int. J. Biol. Macromol. 109: 483-491.
48.Sadasivam, S. and Manickam, A. 1992. Biochemical methods for agricultural sciences. Wiley eastern Ltd, New Delphi, Pp: 184-185.
49.Sarmast, M.K., Salehi, H. and Niazi, A. 2015. Biochemical differences underlie varying drought tolerance in four Festuca arundinacea Schreb. genotypes subjected to short water scarcity. Acta Physiol. Plant. 37: 192.
50.Sheikh-Mohamadi, M.H., Etemadi, N., Arab, M.M., Aalifar, M. and Arab, M. 2018. Physiological and Ascorbate-Glutathione pathway-related genes responses under drought and heatstress in crested wheatgrass. Sci. Hort. 242: 195-206.
51.Sheikh-Mohamadi, M.H., Etemadi, N., Nikbakht, A., Arab, M., Majidi, M.M., and Pessarakli, M. 2017. Antioxidant defence system and physiological responses of Iranian crested wheatgrass (Agropyron cristatum L.) to drought and salinity stress. Acta Physiol. Plant.39: 245.
52.Silva, J.M. and Arrabaca, M. 2004. Contributions of soluble carbohydrates to the osmotic adjustment in the C4 grass Setaria sphacelata: Acomparison between rapidly and slowly imposed water stress. J. Plant Phys. 161: 551-555.
53.Spollen, W.G. and Nelson, C.J. 1994. Response of fructan to water deficit in growing leaves of tall fescue. Plant Phys. 106: 329-336.
54.Szabados, L. and Savoure, A. 2010. Proline: a multifunctional amino acid. Tren. Plant Sci. 15: 2. 89-97.
55.Talukdar, D. 2013. Comparative morpho-physiological and biochemical responses of lentil and grass pea genotypes under water stress. J. Nat. Sci. Biol. Med. 4: 2. 396.
56.Thalmann, M. and Santelia, D.2017. Starch as a determinant of plant fitness under abiotic stress. New Phytol. 214: 3. 943-951.
57.Thalmann, M., Pazmino, D., Seung, D., Horrer, D., Nigro, A., Meier, T., Kolling, K., Pfeifhofer, H.W., Zeeman, S.C. and Santelia, D. 2016. Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants. Plant Cell.28: 8. 1860-1878.
58.Valerio, C., Costa, A., Marri, L., Issakidis-Bourguet, E., Pupillo, P., Trost, P. and Sparla, F. 2011. Thioredoxinregulated beta-amylase (BAM1) triggers diurnal starch degradation in guard cells, and in mesophyll cells under osmotic stress.
J. Exp. Bot. 62: 2. 545-555.
59.Werner, T., Nehnevajov, E., Koellmer, I., Novak, O., Strnad, M., Kraemer, U. and Schmuelling, T. 2010. Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco. Plant Cell. 22: 3905-3920.
60.White, D.A., Turner, N.C. and Galbraith, J.H. 2000. Leaf water relations and stomatal behavior of four allopathic eucalyptus species planted in Mediterranean southwestern Australia. Tree Physiol. 20: 1157-1165.
62.Yang, Z., Xu, L., Yu, J., DaCosta, M. and Huang, B. 2013. Changes in carbohydrate metabolism in two Kentucky bluegrass cultivars during drought stress and recovery. Amer. Soc. Hort. Sci. J. 138: 24-30.
63.Yu, S.M., Lo, S.F. and Ho, T.H.D.2015. Source-Sink Communication: Regulated by Hormone, Nutrient and Stress Cross-Signaling. Tren. Plant Sci. 20: 12. 844-857.
64.Zanella, M., Borghi, G.L., Pirone, C., Thalmann, M., Pazmino, D., Costa, A., Santelia, D., Trost, P. and Sparla, F. 2016. Beta-amylase 1 (BAM1) degrades transitory starch to sustain proline biosynthesis during drought stress. J. Exp. Bot. 67: 6. 1819-1826.
65.Zeeman, S.C., Thorneycroft, D., Schupp, N., Chapple, A., Weck, M., Dunstan, H., Haldimann, P., Bechtold, N., Smith, A.M. and Smith, S.M. 2004. Plastidial alpha-glucan phosphorylase is not required for starch degradation in arabidopsis leaves but has a role in the tolerance of abiotic stress. Plant phys. 135: 2. 849-858
66.Zhang, J. and Kirkham, M.B. 1996. Enzymatic responses of the ascorbategluthathione cycle to drought in sorghum and sunflower plants.Plant Sci. J. 113: 139-147.
67.Zhang, J., Xing, J., Lu, Q., Yu, G., Xu, B. and Huang, B. 2019. Transcriptional regulation of chlorophyll-catabolic genes associated with exogenous chemical effects and genotypic variations in heat-induced leaf senescence for perennial ryegrass. Environ. Exp. Bot. 167: 1-10.
68.Zhang, X. and Ervin, E.H. 2004. Cytokinin-containing seaweed and humic acid extracts associated with creeping bentgrass leaf cytokinins and drought resistance. Crop Sci. J.44: 5. 1737-1745.
69.Zhang, X. and Schmidt, R.E. 1999. Antioxidant response to harmone-containing product in Kentucky bluegrass subjected to drought. Crop Sci. J. 39: 2. 545-551.
70.Zhang, X. and Schmidt, R.E. 2000. Hormone-containing products' impact on antioxidant status of tall fescue and creeping bentgrass subjected to drought. Crop Sci. 40: 5. 1344-1349.
71.Zhang, X., Goatley, M., Wu, W., Ervin, E. and Shang, C. 2019. Drought-induced injury is associated with hormonal alteration in Kentucky bluegrass. Plant Sig. Behav. 14: 10. e1651607.
72.Zhuang, L., Ge, Y., Wang, J., Yu,J., Yang, Z. and Huang, B. 2019. Gibberellic acid inhibition of tilleringin tall fescue involving crosstalkswith cytokinins and transcriptional regulation of genes controlling axillary bud outgrowth. Plant Sci. 287: 110-168.
Journal of Plant Production Research
Volume 27, Issue 2
August 2020
Pages 215-231

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