Study on inheritance of resistance to leaf rust in some bread wheat genotypes by diallel cross

Document Type : scientific research article

Authors

1 M.Sc. Student, Dept. of Agronomy and Plant Breeding, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran,

2 Assistant Prof., Dept. of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Assistant Prof., Faculty Member of Gorgan Agricultural Research Center, Gorgan, Iran

4 Academic member of Gorgan Agriculture Sciences Research Center

Abstract

Background and objectives:
Wheat is attacked by many pathogens during the growing season, including rust. The causative agent of fungal wheat brown rust called Puccinia recondita f. sp. is also known as leaf rust. The most important way to control brown rust disease is to use resistant cultivars that effectively control and reduce its damage. The most important way to control brown rust disease is to use resistant cultivars that effectively control and reduce its damage. The aim of this study was to investigate the inheritance of brown rust resistance in different wheat cultivars and estimation of genetic parameters using diallel crossing.
Materials and methods: Six bread wheat cultivars including Gonbad, Shiroudi, Darya, Atrak and Moghan 3 genotypes with susceptible Bolani cultivar and 15 F1 progenies from one-way diallel crosses were studied. his study was conducted as a randomized complete block design with three replications in field and greenhouse conditions. Traits included infection type, severity of infection, area under disease progression curve and incubation period. Data analysis was performed using SAS ver 9.1 and Excel software and estimation of genetic parameters and heritability of traits were studied by diallel analysis using Griffing's second method with fixed model and Jinks and Hayman method.
Results: Analysis of variance showed that there was a significant difference among genotypes for all traits, indicating genetic variation for these traits and thus genetic analysis could be performed. The mean squares of general and private combining ability were significant for all traits, indicating the importance of both enhancing effects and dominance of genes in inheriting these traits. The Baker ratio indicated the same importance of additive effects and dominance for the incubation period and for the other traits indicated greater importance of additive effects. Estimation of genetic parameters by Jinks and Hayman method showed that for all four traits, the amount of D component was lower than the dominance components (H1 and H2), indicating that the dominance component was more important than the additive component in controlling these traits. The sign of F was positive for all traits, so the frequency of dominant alleles was higher than that of recessive alleles. Wr-vr statistic was significant for all studied traits, indicating the presence of inorganic effects (epistasis). In other words, the significance of this statistic indicates the inaccuracy of the additive-dominance model for traits.
Conclusion: Based on the traits evaluated in field, Atrak cultivar and in greenhouse, Gonbad cultivar had good resistance to brown rust. Narrow-sense heritability of traits was estimated to be between 51 and 86%. Estimation of genetic parameters showed that additive-dominance model was not sufficient. In other words, at least resistance is predictable in resistant cultivars.

Keywords


1.Arzani, A., Ahoon Manesh, A. and Torabi, M. 2005. Study on the genetics of resistance to leaf rust in some bread wheat genotypes at maturity stage. Iranian J. Agric. Sci. 36: 2. 363-373.(In Persian with English Abstract)
2.Dehghani, H., Torabi, M., Moghaddam, M. and Ghannadha, M.R. 2005. Diallel analysis for infection type of yellow rust of wheat. J. Seed Plant. 21: 1. 123-138. (In Persian with English Abstract)
3.Feuillet, C. and Keller, B. 1999. High gene density is conserved at syntentic loci of small and large grass genomes. Proc. Nat. Acad. Sci. USA. 96: 8265-8270.
4.Ghannadha, M.R., Gordon, I.L. and Cromey, M.G. 1995. Diallel analysis of the latent period of stripe rust in wheat. Theor. Appl. Genet. 90: 471-76.
5.Ghannadha, M.R., Soltanloo, H.,Torabi, M. and Ramezanpoor, S.S. 2004. Inheritance of resistance to yellow rust, race 134E182A+, in six wheat cultivars using diallel cross. Iranian J. Agric. Sci.35: 3. 643-656. (In Persian with English Abstract)
6.Kamaluddin, R., Singh, M., Prasad, L.C., Abdin, M.Z. and Joshi, A.K. 2007. Combining ability analysis for grain filling duration and yield traits in spring wheat (Triticum aestivum L. em. Thell.). Genet. Mol. Biol. 30: 411-416.
7.Khan, N.I., Muhammad, S. and Bajwa, M.A. 1994. Combining ability analysis of adult plant yellow and leaf rust resistance in wheat. Pakistan J. Agric. Pp: 70-73.
8.Kheirella, K.A., Defrawy, M. andSherif, T. 1993. Genetic analysis ofgrain yield, biomass and harvest index in wheat under drought stress and normal moisture conditions. Asian. J. Agric. Sci. 24: 163-183.
9.McNeal, F.H., Konzak, C.F., Smith, E.P., Tate, W.S. and Russell, T.S. 1971. A uniform system for recording and processing cereal research data. U.S. Department of Agriculture Research Service. Pp: 34-121.
10.Meghdadzadeh-Kermani, A. 1997. Study on inheritance of resistance to leaf rustin some bread wheat genotypes. M.Sc. Dissertation, University of Karaj.
11.Moeni, R. 1998. Evaluation of disease of wheat and barley and amount of infection and dispread in Zanjan Region. Fani. Press. 52: 12-16. (In Persian with English Abstract)
12.Moeni, R. and Bamdadeian, A. 1994. Distribution of yellow and brown rust disease of wheat in Zanjan Region. 11th Congress of Plant Pathology, Iran, 44p. (In Persian with English Abstract)
13.Nasrollahnejad Ghomi, A.A., Hoseinzadeh, A., Torabi, M. and Ghannadha, M.R. 2003. Study on the genetics of resistance to leaf rust in some advanced lines of wheat at seedling stage. J. Seed Plant. 19: 281-294. (In Persian with English Abstract)
14.Nazan, D. 2008. Genetic analysis of grain yield per spike and some agronomic traits in diallel crosses of bread wheat (Triticum aestivum L.). Turkish J. Agric. For. 32: 249-258.
15.Nicfetrat, A., Taherian, M., Ghanbari, A., Bihamta, M. and Razavi, S.A. 2011. Genetic analysis of resistance to yellow rust of wheat. 6th Congress of Genetics. 2: 13-21. (In Persian with English Abstract)
16.Peterson, R.F., Champbell, A.B. and Hannah, A.E. 1948. Adiagrammatic scale for estimating rust intensity of leaves and stem of cereals. Canadian J. Res. Sec. C: Pp: 26-496.
17.Torabi, M., Nazari, K. and Afshari, F. 2001. Genetics of pathogenicity of Puccinia recondita f. sp. tritici, the causal agent of leaf rust of wheat. Iranian J. Agric. Sci. 32: 3. 625-635.(In Persian with English Abstract)
18.Wilcoxson, R.D., Skovmand, B. and Atif, A.H. 1975. Evaluation ofwheat cultivars for ability to retard development of stem rust. Ann. Appl. Biol. 80: 275-281.
19.Xing, L., Wang, C., Xia, X., He, Z.H., Chen, W., Liu, T., Li, Z. and Liu, D.Q. 2014. Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900. Mol. Breed. 33: 931-937.
20.Yadav, B., Tyagi, C.S. and Singh, D. 1998. Genetical studies and transgressive segregation for field resistance to leaf rust of wheat. Wheat Inf. Serv. 87: 151.
21.Zhang, H., Xia, X.C., He, Z.H., Li, X., Li, Z.F. and Liu, D.Q. 2011. Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Mol. Breed. 28: 527-534.
22.Zhao, X.L., Xia, X.C., He, Z.H., Li,X., Wang, C., Li, Z.F. and Liu,D.Q. 2013. Molecular mapping ofleaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theor. Appl. Gen. 126: 2141-2147.