Evaluation of Some Morphological Traits and Amount of Vinblastine, Vincristine and Ajmalicine Alkaloids in Different Organs of Prewinkle Cultivars (Catharanthus roseus (L.) G. DON)

Document Type : scientific research article

Authors

1 Ph.D. Student of Medicinal Plants, Dept. of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Professor, Dept. of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Associate Prof., Dept. of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

4 Associate Prof., Dept. of Immunology, Faculty of Medical Science, Golestan University of Medical Science, Gorgan, Iran

5 Associate Prof., Dept. of Plant Breeding, Faculty of Plant Production, Gorgan University of Agricultural Siences and Natural Resources, Gorgan, Iran

Abstract

Background and objective: Periwinkle is a plant that is highly valued in the pharmaceutical industry because of its alkaloids. Among the 130 known alkaloids in the genus, Vinblastine and Vincristine alkaloids have particular importance due to their anti-tumor properties. Since Periwinkle is the only source of these compounds, it has a special place among medicinal plants. Due to the low content of indole terpenoids alkaloids in Periwinkle and the importance of these compounds in the pharmaceutical industry, as well as the high economic value of some of its secondary metabolites, six different Prewinkle cultivars were studied for morphological and biochemical traits.
Material and methods: This study was conducted in factorial design based on completely randomized with three replications in field and laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Treatments included six cultivars (Icy Pink, Lilac, Polka Dot, Apricot, Purple, Red Bright) and two organs (leaf and root). The measured factors included fresh and dry weight of roots and leaves, number of lateral stem and root, root length, stem length and amount of Vinblastine, Vincristine and Ajmalicine alkaloids. Alkaloids content were determined by High Performance Liquid Chromatography.
Results: The results showed that the measured parameters had significant effect on morphological and phytochemical properties at 1% probabality level. The highest stem diameter (0.533 cm), root diameter (0.610 cm) and stem length (45.99 cm) were observed in Purple cultivar. The highest amount of Vinblastine was observed in the leaves of Purple (1.7 mg/100g DW) and the lowest level (0.02 mg/100g DW) was measured in the root of Icy Pink. Also the highest amount of Ajmalicine and Vincristine were measured in leaves of Apricot cultivar (1.89 and 3.61 mg/100gDW respectively). However, there were no Ajmalicine alkaloid in the root of Apricot and Red Bright cultivars as well as the leaves of Lilac cultivar.
Conclusion: Different cultivars of Periwinkle have significant differences in the amount of indole terpenoids alkaloids. Although different cultivars of a plant are a subset of the same species, however, the observed differences in different cultivars are controversial. Due to secondary metabolites originate from the primary metabolites of the plant, it is observed that different cultivars of a plant produce different amounts of an alkaloid under the same conditions. This issue can be great importance especially considering the economic value of some alkaloids of this plant. By recommending superior cultivar cultivation in the production of indole terpenoids alkaloids can greatly enhance their production efficiency.

Keywords

References

1.Almagro, L., Fernández-Pérez, F. and Pedreño, M.A. 2015. Indole alkaloids from Catharanthus roseus: bioproduction and their effect on human health. Molecules. 20: 2. 2973-3000.
2.Arora, A. 2013. Phytochemical analysis of methanolic extracts of leaves of some medicinal plants. Biol Forum–An Int J.
5: 2. 91-93.
3.Brezinova, B., Macak, M. and Eftimova, J. 2009. The morphological diversity of selected traits of world collection of poppy genotypes (genus Papaver). Central Europ. Agri. 10: 2. 183-192.
4.Brown B. 2003. Mint soil fertility research in the PNW. Western Nutr. Manage. Conf. 5: 3. 54-60.
5.Chung, I.M., Kim, E.H., Li, M., Peebles, C.A., Jung, W.S., Song, H.K. andSan, K.Y. 2011. Screening 64 cultivars Catharanthus roseus for the production of vindoline, catharanthine, and serpentine. Biotechnol. Prog. 27: 4. 937-943.
6.Darvishzadeh, R. and Hatami, M.H. 2012. Analysis of genetic variation for morphological and agronomic traits in Iranian oriental tobacco (Nicotiana tobaccum L.) genotypes. Plant OMICS Crop Breed. 2: 1. 57-61.
7.Denduangboripant, J., Setaphan, S., Suwanprasart, W. and Panha, S. 2010. Determination of local tobacco cultivars using ISSR molecular marker. Chiang Mai J. Sci. 37: 2. 293-303.
8.Duttagupta, S., Paul, M., Bag, S. and Bhattacharya, M. 2019. Assessment of variation in biochemical characteristics and radical scavenging properties oftwo cultivars of the medical plant Catharanthus roseus. Pharma Innovat. J. 8: 4. 315-317.
9.Essafi, H., Trabelsi, N., Benincasa, C., Tamaalli, A., Perri, E. and Zarrouk, M. 2019. Phytochemical profile, antioxidant and antiproliferative activities of olive leaf extracts from autochthonous Tunisian cultivars. Acta Aliment. 48: 3. 384-390.
10.Gajalakshmi, S., Vijayalakshmi, S. and Devi, R.V. 2013. Pharmacological activities of Catharanthus roseus: a perspective review. Int. J. Pharma Bio. Sci. 4: 2. 431-439.
11.Habibi, H., Mazaheri, D., Majnoon Hoseini, N. and Chaeechi, M.R. 2007. Effect of altitude on essential oil and components in wild thyme (Thymus kotschyanus Boiss.) Taleghan region. Research Construct. 73: 2-10. (In Persian)
12.Hodaei, M., Rahimmalek, M. and Arzani, A. 2017. Variation in morphological characters, chemical composition, and anthocyanin content of different Chrysanthemum morifolium cultivars from Iran. Biochem. Syst. Ecol. 7: 4: 1-10.
13.Idrees, M., Naeem, M. and Khan, M.M.A. 2010. The superiority of cv ‘rosea’ over cv ‘alba’ of periwinkle (Catharanthus roseus L.) in alkaloid production and other physiological attributes. Turk. J. Biol. 34: 1. 81-88.
14.Jacobs, D.I., Snoeijer, W., Hallard,D. and Verpoorte, R. 2004. The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr. Med. Chem. 11: 5. 607-628.
15.Jankulovska, M., Ivanovska, S., Stefkov, G., Acevska, J., Boshev, D. and Jankuloski, L. 2013. Morphological diversity of some opium poppy genotypes (Papaver somniferum L.).In IV Int Symposium “Agrosym.
16.Jordan, M.A. and Wilson, L. 2004. Microtubules as a target for anticancer drugs. Nat. Rev. Cancer. 4: 4. 253-265.
17.Labra, M., Miele, M., Ledda, B., Grassi, F., Mazzei, M. and Sala, F. 2004. Morphological characterization, essential oil composition and DNA genotyping of Ocimum basilicum L. cultivars. Plant Sci. 167: 4. 725-731.
18.Magnotta, M., Murata, J., Chen, J. and De Luca, V. 2006. Identification of a low vindoline accumulating cultivar of Catharanthus roseus (L.) G. Donby alkaloid and enzymatic profiling. Phytochemistry. 67: 16. 1758-1764.
19.Mukherjee, A.K., Basu, S., Sarkar, N. and Ghosh, A.C. 2001. Advances in cancer therapy with plant basednatural products. Curr. Med. Chem.8: 12. 1467-1486.
20.Ndhlala, A., Mulaudzi, R., Ncube, B., Abdelgadir, H., du Plooy, C. and Van Staden, J. 2014. Antioxidant, antimicrobial and phytochemical variations in thirteen Moringa oleifera Lam. cultivars. Molecules. 19: 7. 10480-10494.
21.Née Pal, M.D. and Raychaudhuri, S.S. 2003. Estimation of genetic variability in Plantago ovata cultivars. Plant. Biol. 47: 3. 459-462.
22.Omidbeaigi R. 2013. Production and processing of medicinal plants. Vol. I. Behnashr Press. Mashhad, Iran. 347p. (In Persian)
23.Peyvandi, M. 1992. Investigation of indole alkaloids of three species ofvinca in plant, tissue and cell culture. M.Sc. Thesis, Islamic Azad University, Tehran. (In Persian)
24.Pirkhezri, M., Hassani, M. and Hadian, J. 2010. Genetic diversity in different populations of Matricaria chamomilla l. Growing in southwest of Iran based on morphological. Adv. Med. Plant Res.4: 1. 1-13.
25.Pistelli, L., Najar, B., Giovanelli,S., Lorenzini, L., Tavarini, S. and Angelini, L.G. 2017. Agronomic and phytochemical evaluation of lavandin and lavender cultivars cultivated in the Tyrrhenian area of Tuscany (Italy). Ind. Crops Prod. 109: 37-44.
26.Rai-Dehagi, H., Razmjoo, J., Sabzaliyan, M.R. and Arzani, A. 2014. Effect of shading on morphological characteristics and essential oil content in different of genotypes of three species of mint. Plant Process Funct. 4: 13. 58-69.
27.Sajadi, S.E. and Verpoort, R. 2000. Comparison of 43 cultivars of Periwinkle for Vinblastine anti-cancer alkaloids. Res. Med. Sci. 5: 2. 20-23.(In Persian)
28.Shabani, M., Farsi, M. and Mirshamsi Kakhaki, M. 2014. Evaluation of the effect of ethylene on the expression of T16H, G10H, DAT and AVLBS genes in the Periwinkle (Catharanthus roseus). Mod. Genet. 9: 2. 151-160.
29.Stranska, I., Skalicky, M., Novak,J., Matyasova, E. and Hejnak, V.2013. Analysis of selected poppy (Papaver somniferum L.) cultivars: Pharmaceutically important alkaloids. Ind. Crops Prod. 41: 120-126.
30.Thakore, D., Srivastava, A.K. andSinha, A.K. 2015. Model based fed batch cultivation and elicitation for the overproduction of ajmalicine from hairy roots of Catharanthus roseus. Biochem. Eng. J. 97: 73-80.
31.Xavier, J. and Reddy, J. 2017. AStudy on Antioxidant and antibacterial activities of the fruit and seedextracts of two different cultivars of Momordica charantia L. J. Pharma. Phyto. 6: 6. 1182-1187.
Journal of Plant Production Research
Volume 28, Issue 2
August 2021
Pages 53-66

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