The use of agricultural wastes to produce Iranian isolate of Ganoderma applanatum (Pers.) Pat. and evaluation its performance and some pharmacological properties

Document Type : scientific research article

Authors

1 M.Sc. Student, Dept. of Horticulture and Landscape, Faculty of Agriculture, University of Zabol, Iran

2 . Professor, Dept. of Plant Breeding and Biotechnology, University of Zabol, Iran

3 Ph.D. Student, Dept. of Soil Science, Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources, Iran.

4 Corresponding Author, Assistant Prof. of Horticulture Science (Physiology and Vegetable Breeding), Dept. of Horticulture and Landscape, Faculty of Agriculture, University of Zabol, Iran

5 Associate Prof., Dept. of Plant Protection, Faculty of Agriculture, University of Zabol, Iran

6 Associate Prof., Dept. of Horticulture Engineering, Faculty of Agriculture and Natural Resourses, Imam Khomeini International University, Qazvin, Iran

Abstract

Background and objectives: Selection a suitable substrate and also considering the replacement of other inexpensive agro-wastes instead of valuable wood chips is one of the most important points that should be considered in the cultivation and production of medicinal mushrooms. Selecting the appropriate substrate for fungi and enriching the substrate with organic supplements widely affects the production of fruiting bodies and the medicinal value of fungi.
Materials and methods: This research was carried out in a cultivation hall equipped with temperature, humidity and light control devices at Zabol University and conducted as a two-way factorial based on a completely randomized design with three replicates. Experimental treatments included 10 types of wood chip base substrates (sawdust): Beech tree, Oak wood chips, Hornbeam wood chips, Poplar wood chips, Eucalyptus wood chips, Tamarisk wood chips, Vine sawdust (pruned stems), Date palm sawdust, Date palm leaf sawdust and Sugarcane bagasse. Also, as the second factor, 10% of nitrogenous organic supplements including wheat bran, rice bran, cotton meal and olive pomace were added to each of the main substrates.
Results: The results of this study showed that the highest yield (145.5 gr), total dry matter (36.8 gr), fruit body protein (27.15 mg/100g D.M), ash (4.97 %), Nitrogen (4.38 mg/100g D.M) and total polysaccharide (14.35 mg/g D.M) were related to the combined substrate of oak wood chips with wheat bran supplementation. The highest amount of fungal water (85.16%) was related to the substrate of eucalyptus wood chips and the highest biological efficiency (19.71%) was related to the combined substrate of oak wood chips with olive pomace. The highest potassium (299 mg / 100 g dry matter), calcium (11.73 mg / 100 g dry matter) and antioxidant capacity (54.25%) were recorded for oak wood chips substrate. The results also showed that in the oak wood chip substrate the shortest time in terms of spawn running time (29.40 days), the shortest time for pinhead formation time (43.13 days) and in the hornbeam wood chip substrate the lowest time registered for precocity (70/26 days).
Conclusion: Although the wood chip substrate, especially oak chip was reported as a suitable substrate for growing Ganoderma applanatum, but easy access and lower cost of other agricultural and industrial lignocellulosic wastes make them a suitable alternative. According to the results of this study, the use of combined substrates and organic supplements in the cultivation and production of medicinal fungi, especially Ganoderma applanatum, is recommended.

Keywords

References

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Journal of Plant Production Research
Volume 29, Issue 1
June 2022
Pages 85-109

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