Document Type : original paper
Authors
1
PhD student in Agroecology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
2
- Professor and Associate Professor, College of Agriculture, Ferdowsi University of Mashhad, Iran, respectively.
3
Ferdowsi University of Mashhad, Iran, respectively
4
Faculty of Agriculture & Natural Resources, University of Hormozgan, Bandar Abbas, Iran
Abstract
Statistical models for non-destructive prediction of onion (Allium cepa L.) leaf area
Background and objectives: The importance of rapid, non-destructive, and accurate estimation of leaf area (LA) in agronomic and physiological studies is well known. The need for such estimates of leaf area in particular for leaves with unusual shapes has led to studies relating leaf dimensions and leaf area. However, a search of literature revealed that little information is available for short day onion (Allium cepa L.). The objective of this study was to develop a statistically validated regression model for leaf area prediction from simple non-destructive measurements for onion cultivated in the South of Iran.
Materials and methods: In order to prepare the required plant samples to measure leaf area parameters, an experiment was conducted in the cropping year 2020-2021 in a field of 300 square meters located in Hormozgan province-Rahdad district. The short-day cultivar Takii was used because of its wide planting area in the province. 40 leaves were chosen at random from plants growing in the farm, half were left for validation of the model and the other 20 leaves were subjected to measurement and calibration. The standard method (LICOR LI-3000C) was used for measuring the actual areas of the leaves. Before measuring the leaf area, two main parameters related to leaf length and width were accurately determined for each leaf. To select the best variables and the regression model for estimating leaf area, the fitted simple and multiple linear regression were subjected to the stepwise elimination method. Finally, the accuracy of the selected models was validated using the root mean square error (RMSE) and coefficient of determination (R2).
Results:
All linear models for estimation of leaf area with width (W) and length (L) as well as models including both variables were subjected to regression analysis. The results indicated that leaf length (L) is more appropriate variable for estimating onion leaf area and leaf width (W) was not able to properly describe leaf area. Based on the findings of this research leaf area estimation model with both W and L had the best prediction, with RMSE% = 8.64, RMSE= 1.76 and R2= 0.91. (Area= 0.121 + 0.01537 L2 + 0.3225 L×W).
Conclusion: As the understanding of plant growth and development has been increasing, such mathematical models will be very useful tools for the prediction of leaf area for many plants without the use of expensive devices. Thus, the models from the present study will enable researchers of plant growth modeling to predict leaf area non-destructively with the equations developed.
Keywords: Leaf Area, Leaf Length, Leaf Width, Regression, Coefficient of determination.
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