Comparison of the effect of nitrogen Nano-fertilizer and urea fertilizer on some properties of yew (Taxus baccata L.)

Document Type : scientific research article

Authors

1 M.Sc. Student in Medicinal Plants, Dept. of Horticultural Sciences, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Corresponding Author, Professor, Dept. of Horticultural Sciences, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

3 Ph.D. Graduate in Medicinal Plants, Dept. of Horticultural Sciences, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

Background and objectives: Yew (Taxus baccata L.) is a coniferous plant and one of the plants with multiple medicinal, ornamental and industrial importance is in the process of extinction. Yew is the most important and reliable source of the anti-cancer compound taxol, which has a special importance in medicine. The low amount of taxol in yew and the slow growth of this plant are among the serious obstacles to using yew in providing this important medicinal substance. The use of some elements such as nitrogen will increase the quantitative and qualitative characteristics of plants, especially the production of secondary metabolites such as alkaloids. Based on this, the current research was conducted with the aim of investigating the effect of nanosized nitrogen fertilizer compared to urea fertilizer to increase vegetative growth and metabolite accumulation of yew seedlings.
Materials and methods: For this purpose, an experiment at Gorgan University of Agricultural Sciences and Natural Resources with 9 levels of treatment (nitrogen nanofertilizer at three levels of 0, 1.5 and 3 ml.l-1 in the form of foliar spraying and regular urea fertilizer in three levels of 0, 6 and 12 g were applied to each seedling as soil) and 3 replicates (two plants per replicate). Fertilization was done in 4 stages with a time interval of 14 days. Parameters in two morphophysiological sections (plant height, number of sub-branches, diameter of the main stem, canopy width of the plant, fresh and dry weight of the aerial part and secondary dry weight) and phytochemical (chlorophyll a, chlorophyll b, carotenoid, anthocyanin, total phenol, total flavonoid, percentage of free radical inhibition, total alkaloid and taxol content) measurement became.
Results: The results showed that nitrogen is used in two forms, nanofertilizer and urea, it improved most of the quantitative and qualitative traits of the yew plant. So that the maximum amount of plant height and the diameter of the main stem was obtained with the application of 3 ml.l-1 of nitrogen nanofertilizer along with not using urea. The highest number of sub-branches and amount of chlorophyll a and chlorophyll b were obtained as a result of not using nitrogen nanofertilizer along with 6 g of urea fertilizer. As a result of using 1.5 ml.l-1 of nitrogen nanofertilizer along with 6 g of urea fertilizer, the dry weight of the aerial parts of yew increased significantly. Also, the application of 1.5 ml.l-1 of nitrogen nanofertilizer + 12 g of urea fertilizer produces the highest amount of total alkaloid and taxol in yew.
Conclusion: Based on the results, it seems that it is possible to manage the growth and accumulation of metabolites in the yew by managing nitrogen nutrition and simultaneous application of chelated nitrogen fertilizers and urea fertilizer.

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References

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Journal of Plant Production Research
Volume 31, Issue 3
October 2024
Pages 109-125

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