Effects of superabsorbent nano-polymer, grow-more, iron and zinc fertilizers on morpho-physiological and phytochemical properties of Lavender (Lavandula angustifolia Mill.) under drought stress

Document Type : scientific research article

Author

* Corresponding Author, Research Center of Nutrition and Organic Products (R.C.N.O.P), Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Abstract

Background and objectives: Lavender from the Lamiaceae family is an important plants with many uses in various medicinal and food industrial. Among environmental stresses, water deficit stress plays an important role in the biosynthesis of secondary metabolites in medicinal plants in arid and semi-arid regions like Iran. Drought stress has detrimental effects on growth and yield and can upset the balance between the antioxidant defense system and free radicals by stimulating a series of biochemical reactions, where free radicals cause cell damage. In sustainable production systems of medicinal and aromatic plants, stable quantitative and qualitative functions can be achieved under conditions of environmental stress and it is possible to improve the production of secondary metabolites in these plants under adverse environmental conditions. Therefore, this study was done to evaluate some of important macro-micro nutrients on the morpho-physiological and phytochemical properties of Lavandula angustifolia to determine and introduce the best combinations of fertilizers with use of superabsorbent nano-polymer under water stress conditions.

Materials and methods: This research was using a randomized complete block design (RCBD) in a split-split plot layout with three replications. Irrigation regimes (D1-D3: Field Capacity, 50% and 75% evaporation from basin class A) in main plots, superabsorbent nano-polymer application (S1-S2: use and not use) in sub plots and treatments of fertilizers in sub-sub plots were done. This research was performed in farm of Islamic Azad University Branch of Shahrekord (latitude. 32°20′ N, longitude. 50°51′ E, altitude. 2061 m) in 2023-2024.

Results: Over two consecutive years, the obtained results showed that the use of treatments increased the characters and made the significant effectiveness. In two years, the highest shoot dry matter (61.55 g.plant-1), zinc of shoot (69.55 µg/g dry weight), iron of shoot (82.85 µg/g dry weight), phenol (1.39 mg.Kg Fw-1), chlorophyll content (2.92 mg.g-1 DW) and the lowest amount of prolin (10.89 µg.KgFw-1), made by grow-more fertilizers specially grow-more 1 (20-20-20) in combination with Fe/Zn Libfer. However the superabsorbent nano-polymer application had the aggressive effectiveness on amounts of characters especially in hardness of water stress. In most of measured characters, plants treated with grow-more fertilizers were in the same group. The lowest values for morpho-physiological and phytochemical characters such as shoot dry matter (22.12 g.plant-1), zinc of shoot (28.34 µg/g dry weight), iron of shoot (40.77 µg/g dry weight), phenol (0.29 mg.KgFw-1), chlorophyll content (1.95 mg.g-1 DW) and the most of prolin (32.01 µg.KgFw-1), obtained by plants treated with irrigation regime of 75% evaporation from basin class A (D3) and no application of fertilizers. In some characters, plants treated with F12, F13 or F14 treatments under hardness of drought stress had the same group with control plants. The main constituents of essential oil were Linalool, Linalyl acetate, Limonene, Alpha-pinene, 1, 8 cineole, Borneol, Camphor (alcoholic/cyclic monoterpenes) and Beta-farnesene, Alpha-bisabolene and Beta-Caryophyllene (Sesquiterpenes) that made the most components of essential oil plants. The effect of grow-more 1 (20-20-20) in combination with Fe/Zn Libfer on increasing the total phenol and chlorophyll content, were higher than the other grow-more fertilizers ones.

Conclusion: Generally the application of grow-more fertilizer 1 (20-20-20) with consumption of zinc and iron fertilizers can be a good strategy to improve the morpho-physiological and phytochemical characters of L.angustifolia especially the grow-more 1 (20-20-20) in combination with Fe/Zn Libfer in field capacity water condition. However the use of superabsorbent nano-polymer in strenuous drought stress (75%) is more effectivnes than other water stress levels. Finally, the application of grow-more 1 (20-20-20) in combination with Fe/Zn Libfer can be a good strategy to improve morpho-physiological characters and essential oil quantity and quality of L.angustifolia in cold and semi-arid climates.

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References

1.Mozaffarian, V. (2008). A Pictorial Dictionary of Botanical Taxonomy Latin-English-French-Germany-Persian. Germany: Koeltz Scientific Books.
2.Lamaka, D., Wieczorek, P. P., Bortkiewicz, O., & Paluch, E. (2024). Lavender essential oil market integrity: A comprehensive study of commercial Lavandula angustifolia essential oils adulteration and assessment of industrial standards. Industrial Crops and Products, 222, 119744 https://doi.org/10.1016/ j.indcrop.2024.119744.
3.Magdas, D., Marincas, O., Roncone, A., & Bontempo, L. (2023). Botanical characterization and authentication of lavender essential oil using its volatile organic compounds and compound-specific carbon and hydrogen isotope ratio analysis. Food Control, 154, 110002. https://doi.org/10.1016/j.foodcont.2023.110002.

4.Beknazar Saibnazar, O. R. (2023). Bioecological properties of Lavendula angustifolia Mill. Texas Journal of Multidisiplinary Studies, 21, 1-15.

5.Kharraf, S. E., Guendouz, S. E., Farah, A., Mateus, M. C., El Hadrami, E. M., & Miguel, M. G. (2023). Impact of fifteen combinations of the main components of rosemary, lavender and citrus essential oils on in vitro biological activities. South African Journal of Botany, 156, 162-168.

6.Valchev, H., Kolev, Z., Stoykova, B., & Kozuharova, E. (2022). Pollinators of Lavandula angustifolia Mill., an important factor for optimal production of lavender essential oil. BioRisk, 17, 297-307. https://doi.org/10.3897/ biorisk.17.77364.

7.Camen, D., Hadaruga, N., Luca, R., Doberi, A., Nistor, E., Posta, D., Velicevici, G., Petcov, A., & Sala, F. (2016). Research conserning the influence of fertilization on some physiological processes and biochemical composition of Lavender (Lavandula angustifolia L.). Agriculture and Agricultural Science Prosedia, 10, 198-205. http://creative commons.org/licenses/by-nc-nd/4.0/.

8.Kadam, A. J., Balkate, R. R., Chauhan, A. G., Jadhav, A. A., & Kamble, A. A. (2023). A rewiew on Lavender. International Journal of Current Science, 13(4): 45-58.

9.Askary, M., Behdani, M. A., Parsa, S., Mahmoodi, S., & Jamialahmadi, M. (2018). Water stress and manure application affect the quantity and quality of essential oil of Thymus daenensis and Thymus vulgaris. Industrial Crops and Products, 111, 336-344. https://doi.org/ 10.1016/j.indcrop.2017.09.056.

10.Mumivand, H., Ebrahimi, A., Morshedloo, M. R., & Shayganfar, A. (2021). Water deficit stress changes in drug yield, antioxidant enzymes activity and essential oil quality and quantity of Tarragon (Artemisia dracunculus L.). Industrial Crops and Products, 164, 113381. https://doi.org/10.1016/ j.indcrop.2021.113381.

11.Khorasani, H., Rajabzadeh, F., Mozafari, H., & Ghasemi Pirbalouti, A. (2023). Water deficit stress impairment of morphophysiological and phytochemical traits of Stevia (Stevia rebaudiana Bertoni) buffered by humic acid application. South African Journal of Botany, 154, 365-371. http://dx.doi. org/10.1016/j.sajb.2023.01.030.

12.Babaei, Kh., Moghaddam, M., & Farhadi, N. (2021). Morphological, physiological and phytochemical responses of Mexican marigold (Tagetes minuta L.) to drought stress. Scientia Horticulturae, 284, 110-116. http://dx. doi.org/10.1016/j.scienta.2021.110116.

13.Fariaszewska, A., Aper, J., Van Huylenbroeck, J., & De Swaef, T. (2020). Physiological and biochemical responses of forage grass varieties to mild drought stress under field conditions. International Journal of Plant Production, 14, 335-353. https:// doi.org/10.1007/s42106-020-00088-3.

14.Hayati, A., Rahimi, M. M., Kelidari, A., & Hosseini, S. M. (2021). Effects of humic acid and iron nanochelate on osmolytes content of black cumin (Nigella sativa L.) under drought stress conditions. Journal of Medicinal and Aromatic Plants Research, 37 (5), 809-821. https:// doi.org/10.22092/ijmapr.2021. 354715.2995. [In Persian with English abstract]

15.Caser, M., Chitarra, W., Angiolillod, F., & Perrone, I. (2019). Drought stress adaptation modulates plant secondary metabolite production in Salvia dolomitica Codd. Industrial Crops and Products, 129, 85-96. https://doi.org/ 10.1016/j.indcrop.2018.11.068.

16.Mohsenzadeh, S., Hoseinkhani Hazaveh, M., & Zamanpour Shahmansouri, M. (2020). Evaluation of physiological characters of Lavandula angustifolia L. in response to drought stress, compost and vermicompost fertilizers. Research of Plant Production, 27(3), 149-162. https:// doi.org/ 10.22069/ jopp.2020. 16619.2520.
17.Fascella, G., Angiolilloa, F., Rubertob, G., & Napoli, E. (2020). Agronomic performance, essential oils and hydrodistillation wastewaters of Lavandula angustifolia grown on biochar-based substrates. Industrial Crops and Products, 154, 112733. https://doi.org/10.1016/j.indcrop.2020.112733.

18.Gorgini Shabankareh, H., Khorasani Nejad, S., Soltanlo, H., & Shareati, V. (2021). Effect of ABA on yield, antioxidant enzyms activity and prolin content of Lavandula angustifolia cv. Munstead Organic in response of water deficient. Iran Horticultural Sciences, 52(1), 195-211. [In Persian]

19.Marschner, H. (1995). Mineral Nutrition of Higher Plants. 2nd ed. San Diego: Academic Press: pp: 379-396.
20.Gustafson, A. F. (2010). Handbook of Fertilizers- Their Sources, Make-up, Effects and Use. Read Books. 
21.Malakoti, M. J., & Davodi, M. H. (2002). Zn Forgotten Nutrient in Life Cycle of Plant. Sana issuance, pp 209.
22.Nasiri, Y., Shekari, F., & Asadi, M. (2020). Effect of biofertilizer and zinc sulphate on some morphological characters, yield and essential oil of Satureja hortensis L. Iranian Journal
of Medicinal and Aromatic Plants Research, 36, 523-541. https://doi.org/ 10.22092/ ijmapr. 2020. 341271. 2685.  [In Persian]
23.Mashahiri, Y., Hasanpour Asil, M., Khaledian, M., & Farhangi, M. (2023). Effect of superabsorbent polymer and potassium silicate on morphophysiological and biochemical characteristics of Daffodil (Narcissus tazetta L. cv. Shahla) flower under irrigation regimes. Iranian Journal of Horticultural Science and Technology, 24, 485-502. http:// journal-irshs.ir/article-1-619-en.html. [In Persian with English abstract]
24.Hoseini, A., Heidari, H., Nosrati, A., & Khorami Vafa, M. (2020). Investigating effect of water-superabsorbent polymer on growth and leaf relative water content in foxtail millet (Setaria italica) under drought condition. Crop Science Research in Arid Regions, 2, 231-240. https:// doi.org/ 10.22034/ csrar.2021. 269609.1083. [In Persian]

25.Bagherifard, A., Hamidoghli, Y., Hasan Beiglouei, M., & Orang Ghaedi, M. (2020). Effects of drought stress and superabsorbent polymer on morpho-physiological and biochemical traits of Caper (Capparis spinosa L.). Australian Journal of Crop Science, 14, 13-20. http:// dx.doi.org/ 10.21475/ ajcs. 20. 14. 01.p1418.

26.Dere, S., Güneş, T., & Sivaci, R. (1998). Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany. 22(1), 13-18.
27.Ryan, J., Estefan, G., & Rashid, A. (2001). Soil and Plant Analysis Laboratory Manual. 2nd Ed. Available from ICARDA, Aleppo, Syria.
28.Marinova, D., Ribarova, F., & Atanassaova, M. (2005). Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal University of Chemistry Technology Metallurgy, 40 (3), 255-260.

29.Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060.

30.Adams, R. P. (2007). Identification of Essential Oil Components by Gas Chromatography/ Quadrupole Mass Spectroscopy. Allured Publishing Corporation, Carol Stream, IL.
31.Almrani, H. A. (2019). Effect of chemical fertilizers (NPK) in the growth two species of henbane and total alkaloids content. Iraq Journal of Agriculture, 24(1), 52-61.

32.Amissah, J. N., Alorvor, F. E., Okorley, B. A., Asare, C. M., Osei-Safo, D., Appiah-Opong, R., & Addae-Mensah, I. (2022). Mineral fertilization influences the growth, cryptolepine yield, and bioefficacy of Cryptolepis sanguinolenta (Lindl.) Schlt. Plants, 11(1), 122. https://doi.org/10.3390/plants11010122.

33.Yadegari, M. (2022a). The effects of foliar application of some micronutrient elements on the content and composition of the essential oil of Damask rose (Rosa damascena Mill.). Iran Agricultural Research, 41(2), 35-49. https://doi. org/10.22099/iar.2022.42152.1467.

34.Hamedi, B., Ghasemi Pirbalouti, A., & Rajabzadeh, F. (2022). Responses to morpho-physiological, phytochemical, and nutritional characteristics of Damask Rose (Rosa damescena Mill.) to the applied of organic and chemical fertilizers. Communications in Soil Science and Plant Analysis. 53(17), 1-17. https://doi.org/10.1080/00103624.2022.2070634.

35.Wibowo, S. T. (2007). Kandungan Hormone IAA, Serpan Hara, Dan Pertumbuhan Beberapa Tanaman Budidaya Sebagia Respon Terhadap Aplikasi Pupuk Biologi Bogor: Sekolah Pascasarjana, Institut Pertanian Bogor. 432 pp.
36.Kulak, M. (2020). Recurrent drought stress effects on essential oil profile of Lamiaceae plants: An approach regarding stress memory. Industrial Crops and Products, 154, 1-17. http:// dx.doi.org/ 10.1016/ j.indcrop. 2020.112695.
37.Yadegari, M. (2022c). Effects of NPK, botamisol, and humic acid on morphophysiological traits and essential oil of three Thymus species under drought stress. Iranian Journal of Medicinal and Aromatic Plants Research, 2, 301-321. https://doi.org/ 10.22092/ ijmapr. 2022. 357303. 3119. [In Persian with English abstract]
38.Sasani, N., Pâques, L. E., Boulanger, G., & Singh, A. P. (2021). Physiological and anatomical responses to drought stress differ between two larch species and their hybrid. Trees, 35, 1467-1484. https:// doi.org/ 10.1007/s00468-021-02129-4.
39.Albergaria, E. T., Oliveira, A. F., & Albuquerque, U. P. (2020). The effect of water deficit stress on the composition of phenolic compounds in medicinal plants. South African Journal of Botany, 131, 12-17. http://dx.doi.org/10.1016/ j.sajb.2020.02.002.
40.Alizadeh, A., Moghaddam, M., Asgharzade, A., & Mahmoodi Sourestani, M. (2020). Phytochemical and physiological response of Satureja hortensis L. to different irrigation regimes and chitosan application. Industrial Crops and Products,
158, 112990. https://doi.org/10.1016/ j.indcrop.2020.112990.
41.Yadegari, M. (2022b). Effects of NPK, botamisol, and humic acid on morphophysiological traits and essential oil of three Satureja species under drought stress. Iranian Journal of Medicinal and Aromatic Plants Research, 38 (1), 61-80. https://doi.org/ 10.22092/ ijmapr. 2022. 356264. 3073. [In Persian with English abstract]

42.Han, R. C., Xu, Z. R., Li, C. Y., Rasheed, A., Pan, X. H., Shi, Q. H., & Wu, Z. M. (2022). The removal of nitrate reductase phosphorylation enhances tolerance to ammonium nitrogen deficiency in rice. Journal of Integrative Agriculture, 21(3), 631-643. http://dx.doi.org/10.1016/S2095-3119 (20)63473-6.

43.Fathi, B., Reezi, S., Rouhi, V., & Ghobadi Neya, M. (2017). Effect of different levels of superabsorbent and vermicompost on qualitative and quantitative traits of gazania (Gazania hybrida) in green roof condition. Journal of Horticultural Science,
48, 423-429. https://doi.org/10.22059/ ijhs.2017.206858.1011. [In Persian with English abstract]
44.Thakur, M., & Kumar, R. (2020). Foliar application of plant growth regulators modulates the productivity and
chemical profile of Damask rose (Rosa damascena Mill.) under mid hill conditions of the western Himalaya. Industrial Crop Products, 158, 113024. https://doi.org/10.1016/j.indcrop.2020.113024.
45.Mahmodi, A., Yadegari, M., & Hamedi, B. (2020). Effect of Vermi-compost, nitrogen and phosphorus fertilizers on morphological and phytochemical properties of Mentha piperita L. Journal of Plant Environmental Physiology,
15, 84-99. https://dorl.net/dor/20.1001. 1.76712423.1399.15.57.7.0. [In Persian with English abstract]

46.Bani Taba, A., Yadegari, M., & Naderi Darfaghshahi, M. R. (2022). Effect of irrigation regimes and foliar application of manganese and copper on morphophysiological traits of three species of Saturjea sp. in Isfahan and Golpayegan regionsl. Plant Process and Function, 11, 129-147. https://doi.org/ 10.1016/j.jssas.2024.05.003. [In Persian with English abstract]

47.Babalar, M., Mohtashami, S., Tabrizi, L., & Roushan, V. (2020). Effect of ammonioum sulfat on yield properties and phytochemical properties of Savory (Satureja hortensis L. Saturn). Journal of Horticultural Science, 51, 273-286. https://dorl.net/dor/20.1001.1.2008482.1399.51.2.1.7. [In Persian]

48.Aghaei, K., Ghasemi Pirbalouti, A., Mousavi, A., Naghdi Badi, H. A., & Mehnatkesh A. M. (2021). Effects of different fertilizers and the foliar application of L-phenylalanine on mineral contents of hyssop [Hyssopus officinalis L. subsp. angustifolius (Bieb.)]. Journal of Horticultural Plants Nutrition, 4(2), 13-28. https://doi.org/10. 22070/hpn.2021.4639.1038. [In Persian with English abstract]

49.Asgarian, M., Aminifard, M. H., Khayat, M., & Jahani, M. (2019). Evaluation of different level of complete fertilizer NPK and folic acid on morphological properties, yield and yield components of Ocimum basilicum L. Journal of Agroecology, 11, 1375-1388. https:// doi.org/ 10. 22067/ jag. v11i4. 73422. [In Persian with English abstract]

50.Yadegari, M. (2024a). The effects of foliar application of some important micronutrient elements on essential oil content and components of Dracocephalum kotschyi Boiss. Journal of Horticulture and Postharvest Research, 7(1), 81-98. https://doi.org/ 10.22077/jhpr.2024.7079.1351.
51.Yadegari, M. (2024b). Effects of some biological and chemical fertilizers on morphophysiological characters and essential oil in Rosa damascena Mill. in drought stress. Journal of Plant Process and Function, 12, 417-440. https:// doi.org/ 10.22034/ 12.57.417.[In Persian with English abstract]
52.Javadi, H., Mousavi, Gh., Seghatoleslami, M. J., & Kermani, F. (2021). Effect of organic and chemical improvers application on yield and essential oil percentage of Dill (Anethum graveolens L.) under water deficit
stress conditions. Plant Productions, 44, 283-294. https://doi.org/10.22055/ ppd.2019.30621.1806. [In Persian with English abstract]

53.Beiranvandi, M., Akbari, N., Ahmadi, A., Momivand, H., & Nazarian F. (2020). Interaction of biochar and superabsorbent on the composition of Satureja rechingeri Jamzad essential oil under drought stress. Iranian Journal of Medicinal and Aromatic Plants Research, 36, 780-793. https://doi.org/10.22092/ ijmapr.2020.342067.2732. [In Persian with English abstract]

54.Rezaee, Z., & Rafeol Hoseini, M. (2017). Physiological response of Foeniculum vulgare Mill. to manuare fertilizer and superabsorbent under drought stress. Ecophysiology of Crop Science, 43, 547-564. https://sanad.iau. ir/Journal/jcep/Article/956799/FullText.
55.Pirzad, A., Darvishzadeh, R., & Hasani, A. (2017). Seed yield and essential oil responses of Cumin to different irrigation regimes and super absorbent levels in Urmia climatic conditions. Crop Science Research in Arid Regions, 1, 1-12. https://doi.org/10.22034/csrar. 01.01.01. [In Persian with English abstract]

56.Danesh-Shahraki, H., Ghasemi Pirbalouti, A., & Rajabzadeh, F. (2023). Water deficit stress mitigation by the foliar spraying of salicylic acid and proline on the volatile oils and growth features of hyssop (Hyssopus officinalis L.). Journal of Essential Oil-Bearing Plants, https://doi.org/10.1080/0972060X.2022.2160279.

57.Esch, E. H., Lipson, D. A., & Cleland, E. E. (2019). Invasion and drought alter phenological sensitivity and synergistically lower ecosystem production. Ecology, 100, 34-45.‏ https://doi.org/10.1002/ ecy.2802.
58.Nosrati, M., Zeidalai, A., Zare, M. J., Momivand, H., & Keyani, M. (2021). Effect of sowing time, complete fertilizer and harvesting time on qualitative and quantitative yield of Satureja rechingeri Jamzad. Iranian Journal of Medicinal and Aromatic Plants Research, 37, 242-259. https:// doi.org/10.22092/ijmapr.2021.341804.2716. [In Persian with English abstract]
Journal of Plant Production Research
Volume 33, Issue 2
September 2026
Pages 1-29

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