Effects of different culture media and growth regulators on micropropagation of blueberry (Vaccinium corymbosom L. cv. Bluegold)

Document Type : scientific research article

Authors

1 Ph.D. Student of Dept. of Horticultural Sciences, Dept. of Horticultural Sciences, University Campus 2, University of Guilan, Rasht, Iran

2 Corresponding Author, Assistant Prof., Dept. of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Prof., Dept. of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Associate Prof., Dept. of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

Background and objectives:
The blueberry plant belongs to the Ericaceae family and the Vaccinium genus, which is native to North America. In recent years, the demand for blueberry consumption has increased significantly due to the presence of valuable antioxidant compounds such as flavonoid, anthocyanin and phenolic acids and the effect on human health. The use of new methods of plant propagation such as tissue culture can be effective in the mass production of blueberry cultivars, garden construction and finally blueberry fruit production. Although blueberry cultivation has not been carried out on a large scale in Iran, there are environmental requirements for its growth and commercial production in some regions of Iran.
Materials and methods:
In this research, lateral shoot explants with two nodes of Bluegold cultivar of blueberry were prepared and after disinfection in Murashige and Skoog (MS), Woody Plant Medium (WPM) and Anderson (AN) basic culture media containing Zeatin growth regulators. , TDZ, 2iP and BA were cultured at three levels (0.5, 1 and 1.5 mg/L) for proliferation. After two months, new branches were grown and fertilized, and the indices of shoot length, number of shoots, number of leaves, and chlorophyll a and chlorophyll b were measured in each explant. Rooting potential on the shoots produced in the mentioned culture medium along with the growth regulators of IBA, NAA and IAA at three levels (1, 2 and 3 mg/L) based on the complete randomly design and in three replicate, was performed. After 8 weeks, the characteristics of rooting percentage, root length and number of roots were evaluated.
Results:
In the processing stage after 10 weeks, the desired traits were measured, the longest shoot length (4.93 cm) and the highest number of leaves (18.75) in the treatment of WPM culture medium with Zeatin hormone, the highest number of shoots (8.22) in the application of Zeatin at the rate of 1.5 mg/l, the highest amount of chlorophyll a (1.71 mg/g) in MS culture medium and chlorophyll b (0.36 mg/g) It was observed in the treatment of WPM culture medium with Zeatin. In the rooting stage after 8 weeks, the desired traits were measured, the highest number of roots (3.64) and the longest root length (8.22 cm) with the application of IBA in the amount of 2 mg/l in the environment. WPM cultivation and the highest percentage of rooting (67.11) was observed with the application of IBA at the rate of 2 mg/l.
Conclusion:
In general, the results of this research showed that in the processing test, the use of 1.5 mg/L of zeatin in the AN culture medium has effective effects on the bluegold blueberry proliferation. In the rooting test, the use of 2 mg/L IBA in WPM culture medium with half the concentration of macro substances showed better results than other growth regulators and other culture mediums. Therefore, the above treatments can be suggested for processing and rooting of Bluegold blueberry.

Keywords

Main Subjects

References

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Journal of Plant Production Research
Volume 33, Issue 1
April 2026
Pages 63-79

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