نوع مقاله : پژوهشی
نویسندگان
1 استادیار پژوهش موسسه تحقیقات برنج کشور- معاونت مازندران. سازمان تحقیقات، آموزش و ترویج کشاورزی، آمل، ایران
2 استاد دانشگاه شهید چمران اهواز-
3 دانشیار دانشگاه شهید چمران اهواز
4 دانشیار گروه زراعت، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان دانشگاه علوم کشاورزی و منابع طبیعی ساری
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Introduction:
Water deficit in soil and atmosphere are the most important limiting factor for photosynthesis and plants growth especially rice and negative effect are created on photosynthesis through absorption carbon decrees that is caused stomatal and metabolic constraints. Since the capacity for photo protection is limited, drought can lead to damaged and loss of active PSII reaction centers. Therefore, non-destructive measurements of photosynthesis by chlorophyll a fluorometry may potentially provide to plant viability and performance in response to drought. Rice sensitive to drought increase when drought happen during the vegetative and flowering period. plants response to drought stress by identification of character that an important role in drought tolerance can be assessment in levels of morphological, cellular, biochemical and molecular. This experiment was conducted to evaluate the rice genotypes by measuring the traits associated with photosynthetic pigments and chlorophyll fluorescence components in order to select appropriate criteria for screening and drought stress tolerant genotypes
Materials and Methods:
This experiment was conducted completely randomized design in two condition of well watered and drought stress during seedling stage in rice genotypes with 56 different provinces of Iran and the International Rice Research Institute (IRRI) in Iran Rice Research Institute (Amol) in 2014. After seeding, irrigation was carried out on a daily basis up to two to three leaf seedling stage. After 15 days, in Customary irrigated conditions, irrigation continued to end of the growth period and was completely cut off under irrigation stress. After soil moisture reached 20% (about 10 days after irrigation stop), the traits of photosynthetic pigments such as chlorophyll a, b and carotenoids and chlorophyll fluorescence components such as Fv / Fm, ΦPSII, q N, ETR and PAR was measured on the basis of scientific methods
Result and Discussion:
The results showed that the amount of chlorophyll a and b, respectively, 2.3 and 21.4 percent increase and carotenoids content, 3.8 percent decline in drought stress. Chlorophyll fluorescence parameters except Fv: Fm showed significant differences in the two environments. ΦPSII, qN, ETR and PAR parameters, respectively, the value of 7.3, 13, 35.5 and 28.8 percent were reduced in stress to normal condition. Correlation results showed that chlorophyll a with b and carotenoids with chlorophyll a and b had significant positive correlation. Correlation between ΦPSII with Fv:Fm and qN to correlation coefficient 0.29 and - 0.91 was significant, respectively, .
Conclusions:
The results showed that under drought stress can be using chlorophyll pigments and chlorophyll fluorescence parameters, especially ΦPSII and ETR as evaluation criteria for the selection and screening of tolerant rice genotypes utilized
کلیدواژهها [English]