The influence of B5 basal medium on morphological behavior of Lily (Lilium longiflorum) bulbscale in vitro

This study was conducted to investigate the effects of in vitro and regeneration of lily (Lilium longiflorum) bulb scale by different plant growth regulators in B5 medium in a completely randomized design with factorial arrangement and 4 replications. Treatments consisted of indole-3-acetic acid (IAA) and 2,4-D in B5 medium in four concentrations (0, 0.5, 1, 2 mg/lit) and these were combined to medium. Evaluation of results indicated that with increasing of IAA concentration, number of bulblets increased significantly but between using of various IAA concentrations and rooting parameters and callus formation, no significant difference was observed. With increasing of 2,4-D concentration to 2 ppm, callus fresh weight was enhanced, whereas with increasing of 2,4-D concentration, organogenesis suppressed completely. According to the results, about 100-150 plantlet were achieved per adult bulb of lily.