ارزیابی میوه های تمشک سیاه خاردار و بی خار از نظر ویژگی های فیزیکوشیمیایی در زمان بلوغ و طی انبارمانی

نوع مقاله : مقاله کامل علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد باغبانی، گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 نویسنده مسئول، استادیار گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 دانشیار گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

4 کارشناس‌ارشد باغبانی، گروه علوم باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

چکیده
سابقه و هدف: تمشک سیاه (Rubus sp.) از خانواده گل سرخیان، میوه‌ای است که به‌دلیل عطر و طعم منحصر به فرد و مقادیر بالایی از ترکیب های آنتی اکسیدان از ارزش زیادی برخوردار است. تمشک سیاه دارای طیف وسیعی از ارقام به طور کامل بی خار تا خاردار با خارهای بسیار متراکم با مقادیر مختلف اندازه و انحنا می باشد. میوه های تمشک پس از برداشت به‌دلیل سرعت تنفس بالا به‌سرعت از بین می روند. با توجه به ارزش غذایی و نیاز روزافزون بازار مصرف، در تحقیق حاضر ویژگی های فیزیکوشیمیایی میوه تمشک سیاه خاردار و بی‌خار در زمان رسیدن و طی نگهداری مورد ارزیابی قرار گرفت.
مواد و روش‌ها: میوه‌های تمشک سیاه خاردار (Rubus fruticosis) و بی‌خار (R. laciniantus) در زمان رسیدن تجاری برداشت شده و از نظر ویژگی‌های فیزیکی در قالب طرح کاملا تصادفی در سه تکرار با هم مقایسه شدند. به‌علاوه آزمایشی به‌صورت فاکتوریل با دو فاکتور نوع تمشک سیاه (خاردار و بی‌خار) و دوره انبارمانی (صفر، چهار و هشت روز) در قالب طرح کاملا تصادفی با سه تکرار انجام شد. به این منظور حدود ۱۰۰ گرم میوه تمشک سیاه خاردار و بی‌خار در هر تکرار در ظروف پلی‌استایرن منفذدار قرار داده شد و در دمای ۴±۱ درجه سانتی‌گراد نگهداری گردید. ویژگی‌های فیزیکوشیمیایی میوه‌ها در زمان برداشت (روز صفر) و در روزهای چهارم و هشتم انبارمانی اندازه‌گیری شد.
یافته‌ها: نتایج نشان داد که میوه تمشک سیاه خاردار نسبت به بی‌خار در زمان رسیدن طول، قطر، ویتامین‌ث، مواد جامد محلول کل، اسیدیته، شاخص طعم، آنتوسیانین، فعالیت آنتی‌اکسیدانی و فنل کل بیشتری داشت در حالی‌که اسید قابل تیتر در تمشک بی‌خار بالاتر بود. با افزایش طول دوره انبارمانی مواد جامد محلول کل، اسیدیته و شاخص طعم به‌طور معنی‌داری افزایش پیدا کرد ولی ویتامین‌ث و اسید قابل تیتر کاهش یافت. همچنین میزان بالاتر کاهش وزن در روز هشتم انبارداری در تمشک بی‌خار (۵۴/۹ درصد) نسبت به تمشک خاردار (۱۶/۲ درصد) ثبت شد. در هر دو نوع تمشک، بیشترین میزان آنتوسیانین در روز چهارم انبارداری مشاهده شد و میزان آنتوسیانین با افزایش مدت نگهداری، کاهش یافت. در تمشک خاردار و بی‌‌خار با افزایش دوره انبارمانی ابتدا میزان فعالیت آنتی‌اکسیدانی افزایش پیدا کرد و سپس تا روز هشتم انبارمانی میزان فعالیت آنتی‌اکسیدانی کاهش پیدا کرد که این کاهش در تمشک بی‌خار بیشتر از تمشک خاردار بود. میزان فنل کل در تمشک خاردار با افزایش زمان انبارمانی تا روز چهارم کاهش پیدا کرد و از روز چهارم تا روز هشتم انبارمانی میزان فنل کل افزایش یافت، اما در تمشک بی‌خار بر خلاف آن مشاهده شد به‌طوری‌که با افزایش زمان انبارمانی تا روز چهارم میزان فنل کل افزایش یافت و پس از آن تا روز هشتم میزان فنل کل کاهش نشان داد.
نتیجه‌گیری: در مجموع، تمشک سیاه خاردار کیفیت بالاتری در زمان رسیدن و ماندگاری بهتری در طی انبارمانی نشان داد.
واژه‌های کلیدی: آنتوسیانین، شاخص طعم، فعالیت آنتی‌اکسیدانی، فنل کل

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of Physicochemical Traits of Thorny and Thornless Blackberries at Ripening and During Storage

نویسندگان [English]

  • Farank Yegane 1
  • Feryal Varasteh 2
  • Mahdi Alizadeh 3
  • Yousef Ghasemi 4
1 M.Sc. Student of Horticulture, Dept. of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Corresponding Author, Assistant Prof., Dept. of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3 Associate Prof., Dept. of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
4 M.Sc. of Horticulture, Dept. of Horticulture, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

Abstract
Background and objectives
Blackberry (Rubus sp.) from Rosacea family is a fruit that has a high value due to its unique taste and high amounts of antioxidant compounds. Blackberries contain a wide range from completely thornless to thorny cultivars with highly dense spines of varying degrees of size and curvature. Blackberry fruits decay rapidly after harvest due to high respiration rate. Considering the nutritional value and increasing needs of the consumer market, in the present research physicochemical traits of thorny and thornless blackberries at ripening and during storage were evaluated.
Materials and methods
Thorny and thornless blackberry fruits were harvested at commercial maturity and were compared in terms of physical characteristics in the form of a completely randomized design in three replications. In addition, a factorial experiment was conducted with two factors of blackberry type (thorny and thornless) and storage period (zero, four and eight days) in the form of a completely randomized design with three replications. For this purpose, about 100 grams of thorny and thornless blackberries were placed in porous polystyrene containers and kept at a temperature of 4±1 ˚C. The physicochemical characteristics of fruits were measured at the time of harvest (day zero) and on the fourth and eighth days of storage.
Results
The results showed that at ripening stage, thorny blackberries had higher fruit length, diameter, vitamin C, soluble solids, pH, flavor index, anthocyanin, antioxidant activity and total phenol than thornless blackberries, while titratable acidity was higher in thornless blackberries. Increasing the number of thorns by expanding the photosynthetic surface, such as vegetative traits of leaflet width, diameter and number of branches, seems to increase carbohydrate production in thorny plants, which are also used to produce secondary metabolites and phytoalexins in the plant and provides reduction of damage from pests and diseases. With increasing the storage period, soluble solids, pH, flavor index, total phenol were increased significantly, but the antioxidant activity, vitamin C and titratable acidity were decreased. Also, the highest rate of fruit weight loss (9.54%) was recorded on the eighth day of storage in thornless blackberries. In both types of blackberries, the highest amount of anthocyanin was observed on the fourth day of storage, and the amount of anthocyanins in both thorny and thornless blackberries was decreased with increasing storage time, on the eighth day of storage as compared to the fourth day. In thorny and thornless blackberries, with the increase of storage period, at first days the amount of antioxidant activity was increased and then until the eighth day of storage, the amount of antioxidant activity was decreased, which was more in thornless blackberries than thorny blackberries. The amount of total phenol in thorny blackberries was decreased with increasing storage time until the fourth day of storage and then to the eighth day of storage the amount of total phenol was increased, however in thornless blackberries the situation was vice versa so that with increasing storage time to day fourth, the amount of total phenol was increased and then until the eighth day, the amount of total phenol was decreased.
Conclusion:
Overall, thorny blackberries had a higher quality at the time of ripening and better shelf life during storage.
Keywords: Anthocyanin, Antioxidant activity, Flavor index, Total phenol

کلیدواژه‌ها [English]

  • Anthocyanin. Antioxidant activity
  • Flavor index
  • Total phenol
1.Yousefi, Q., Sadeghi, S., Karami, Z., Imam Juma, Z. & Jookey, M. (2014). Evaluation of the effect of modified atmospheric packaging on the shelf life and keeping quality of raspberry using RSM. Journal of Food Technology and Nutrition, 11(4), 45-56.
2.Alizadeh, M. & Nazari, J. (2021). An introduction to the wild and feral fruits of Golestan province. Gorgan University of Agricultural Sciences and Natural Resources Press. 557 p. [In Persian]
3.Kaume, L., Howard, L. R. & Devareddy, L. (2012). The blackberry fruit: a review on its composition and chemistry, metabolism and bioavailability, and health benefits. Journal of Agricultural and Food Chemistry, 60, 5716-5727.
4.Tulio, J. R., Reese, R. N., Wyzgoski, F. J., Rinaldi, P. L., Fu, R., Scheerens, J. C. & Miller, A. R. (2008). Cyanidin 3-rutinoside and cyanidin 3-xylosylrutinoside as primary phenolic antioxidants in black raspberry. Journal of Agricultural and Food Chemistry, 56, 1880-1888.
5.Haddadinejad, M., Ghasemi Omran, S. & Azimi Ahangari, F. (2015). Morphological diversity of black raspberries in some areas of Mazandaran province. Iranian Journal of Horticultural Science, 46(2), 333-343. [In Persian]
6.Clark, J. R. & Finn, C. E. (2011). Blackberry breeding and genetics. In: Flachowsky, H., Hanke, V. M (Eds), Methods in Temperate Fruit Breeding. Fruit, Vegetable and Cereal Science and Biotechnology, 5(1), 27-43.
7.Khatamsaz, M. (1998). The Flora of Iran, rosaceae. Research institute of forests and pastures. 352 p. [In Persian]
8.Haddadinejad, M. & Moradi, H. (2016). Evaluation of genetic diversity of some Iranian black berries based on morphological traits. Iranian Journal of Horticultural Science, 47(2), 371-382. [In Persian]
9.Jafari, Z. & Gharaghani, A. (2012). Study of growth and yield of some blackberries species from throughout Iran in Bajgah. M.Sc. Thesis. Department of horticulture, University of Shiraz. [In Persian]
10.Han, C., Zhao, Y., Leonard, S. W. & Traber, M. G. (2004). Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria × ananassa) and raspberries (Rubus ideaus). Postharvest Biology and Technology, 33, 67-78.
11.Bower, C. (2007). Postharvest handling, storage, and treatment of fresh market berries. In: Yanyun Zhao (Ed.), Berry fruit: Value-added products for health promotion. USA, Corvallis: Taylor &Francis Group, CRC Press. 442 p.
12.Funt, R. C. (2013). Growth and development. In: R. C. Funt and Harvey K. H. (Eds), Raspberries. CABI. Pp: 83-90.
13.Haddadinejad, M., Ghasemi, K. & Mohammadi, A. A. (2018). Effect of storage temperature and packaging material on shelf life of thornless blackberry. International Journal of Horticultural Science and Technology, 5(2), 265-275.
14.Contreras, C., Adolfo, H. & Élida, C. (2021). Postharvest physiology and storage potential of new Chilean raspberry cultivars. Agricultural Research Journal, 81, 144-169.
15.Rezaee Kivi, A., Sartipnia, N. & Babai Khalkhali, M. (2014). Effect of storage temperatures on antioxidant capacity and bioactive compounds in raspberry fruit. International Journal of Plant, Animal and Environmental Sciences, 4(3), 343-349.
16.Varasteh, F., Arzani, K., Barzegar, M. & Zamani, Z. (2017). Pomegranate (Punica granatum L.) fruit storability improvement using pre-storage chitosan coating technique. Journal of Agricultural Science and Technology, 19, 389-400.
17.Manolopoulou, H. & Papadopoulou, P. (1998). A study of respiratory and physicochemical change of four Kiwifruit cultivars during cold-storage. Food Chemistry, 63, 529-534.
18.Wagner, G. J. (1979). Content and vacuole/ extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64, 88-93.
19.Slinkard, K. and Singleton, V.L. (1977). Total phenol analysis. automation and comparison with manual methods. The American Journal of Enology and Viticulture, 28, 49-55.
20.Miliauskas, G., Venskutonis, P. R. & Vanbeek, T. A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry, 85, 231-237.
21.Strik, B., Mann, J. & Finn, C. (1996). Percent drupelet set varies among blackberry genotypes. The Journal of the American Society for Horticultural Science, 121, 371-373.
22.Mohammadi, A. A., Norooz Valashedi, R. & Hadadinejad, M. (2020). Evaluation of heat requirement and growth parameters of three cultivars of Blackberry (Rubus sp.) under climatic conditions of Sari, Iran. Journal of Agricultural Meteorology, 8(2), 26-34. [In Persian]
23.Vargas, M., Albors, A., Chiralt, A. & Gonzalez-Martinez, C. (2006). Quality of cold-stored strawberries as affected by chitosan-oleic acid edible coatings. Postharvest Biology and Technology, 41, 164-71.
24.Rahmanzadeh Ishkeh, Sh., Asghari, M. R., Shirzad, H. & Alirezalu, A. (2021). Evaluation of antioxidant activity and phytochemical constituents of raspberry fruit (Rubus ulmifolius sub sp. sanctus) collected from Khan Daracy region of Urmia, west Azerbaijan province of Iran. Eco-phytochem. The Journal of Medicinal of Plants, 32(4), 89-101. [In Persian]
25.Giongo, L., Matteo, A. & Paula, P. (2019). Raspberry texture mechanical profiling during fruit ripening and storage. Postharvest Biology and Technology, 149, 177-186.
26.Shokrollah Fam, S., Hajilou, J., Zare, F., Tabatabaei, S. J. & Naghshiband Hasani, R. (2012). Effects of calcium chloride and salicylic acid on quality and shelf life of plum "golden drop" cultivar. Journal of Food Research, 22(1), 75-85. [In Persian]
27.Yaman, O. & Bayoindirli, L. (2002). Effects of an edible coating and cold storage on shelf life and quality of cherries. LWT - Food Science and Technology, 35, 146-150.
28.Piga, A. D., Aquino, S. & Agabbio, M. (2000). Influence of cold storage and shelf-life on quality of Salustiana, orange fruits. Fruits, 55, 37-44.
29.Gao, P., Zhu, Z. & Zhang, P. (2013). Effects of chitosan–glucose complex coating on postharvest quality and shelf life of table grapes. Carbohydrate Polymers, 95, 371-378.
30.Kafkas, E., Kosar, M., Turemis, N. & Baser, K. H. C. (2006). Analysis of sugars, organic acids and vitamin C contents of blackberry genotypes from Turkey. International Journal of Food Chemistry, 97(4), 732-736.
31.Sapei, L. & Hwa, L. (2014). Study on the kinetics of vitamin C degradation in fresh strawberry juices. Procedia Chemistry, 9, 62-68.
32.Arena, E., Fallico, B. & Maccarine, E. (2001). Evaluation of antioxidant capacity of blood orange juices as influenced by constituent’s concentration process and storage. Food Chemistry, 74, 423-427.
33.Piechowiak, T., Piotr, A., Patryk, K. & Karol, S. (2019). Impact of ozonation process on the microbiological and antioxidant status of raspberry (Rubus ideaeus L.) fruit during storage at room temperature. Agricultural and Food Science, 28(1), 189-208.
34.Emamifar, A., Kadivar, M., Shahedi, M. & Soleimanian-Zad, S. (2010). Evaluation of nanocomposite packaging containing Ag and ZnO on shelf life of fresh orange juice. Innovative Food Science & Emerging Technologies, 11(4), 742-748.
35.Vaezi, S., Asghari, M., Farrokhzad, A. & Yousefi, Z. (2021). The effect of silver and silica nanocomposite packaging on the quality properties and enzymatic activity of freshly cut fruit of Red Gold cultivar. Pomology Research Journal, 6(1), 1-9. [In Persian]
36.Zheng, X., Tian, S.H., Meng, X. & Li, B. (2007). Physiological and biochemical responses in peach fruit to oxalicacid treatment during storage at room temperature. Food Chemistry, 104, 156-162.
37.Perkins-Vaezie, P. (2007). Blueberry fruit response to postharvest application of ultraviolet radiation. Postharvest Biology and Technology, 10, 1005-1016.
38.Karabulut, O. A., Cohen, L., Wiess, B., Daus, A., Lurie, S. & Droby, S. (2002). Control of brown rot and blue mold of peach and nectarine by short hot water brushing and yeast antagonists. Postharvest Biology and Technology, 24, 103-111.
39.Varasteh, F., Arzani, K., Barzegar, M. & Zamani, Z. (2012). Changes in anthocyanins in arils of chitosan-coated pomegranate (Punica granatum L. cv. Rabbab-e-Neyriz) fruit during cold storage. Food Chemistry, 130, 267-272.
40.Vattem, D. A., Ghaedian, R. & Shetty, K. (2005). Enhancing health benefits of berries through phenolic antioxidant enrichment: focus on cranberry. Asia Pacific Journal of Clinical Nutrition, 14(2), 120-130.
41.Michalak, A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress.
Polish Journal of Environmental Studies,
15(4), 523-530.
42.Ghorbani, A., Bakhshi, D., Haj Najari, H., Ghasemnejad, M. & Taghi Dost, P. (2011). Phenolic compounds and antioxidant activity of some Iranian and imported cultivars of apple in Karaj region. Journal of Horticultural Sciences, 24(1), 83-90. [In Persian]
43.Shoji, T. (2007). Polyphenols as natural food pigments. changes during food processing. American Journal of Food Technology, 2, 570-581.
44.Perkins-Veazie, P. & Kalt, W. (2002). Postharvest storage of blackberry fruit does not increase antioxidant levels. Acta Horticulturae, 585, 521-524.
45.Hassimotto, N. M. A., Mota, R. V. D., Cordenunsi, B. R. & Lajolo, F. M. (2008). Physico-chemical characterization and bioactive compounds of blackberry fruits (Rubus sp.) grown in Brazil. Journal of Food Science and Technology, 28(3), 702-708.
46.Dadkhah Aghdash, H., Daemi Saeidabad, M. & Falahati Anbaran, M. (2019). The Total phenolic content, total flavonoids and the antioxidant capacity in two wild species of raspberry, Rubus persicus and R. caesius, at different maturity stages of fruits. Plant Productions, 42(3), 295-306. [In Persian]
47.Kim, M. J., Perkins-Veazie, P., Guoying, M. & Fernandez, G. (2015). Shelf life and changes in phenolic compounds of organically grown blackberries during refrigerated storage. Postharvest Biology and Technology, 110, 257-263.