نوع مقاله : مقاله کامل علمی پژوهشی
نویسندگان
1 دانشیار گروه بیولوژی، دانشگاه پیام‌نور، تهران، ایران.
2 نویسنده مسئول، دانشیار گروه کشاورزی، دانشگاه پیام‌نور، تهران، ایران.
3 کارشناسی‌ارشد زیست‌شناسی سلولی ملکولی، دانشگاه خوارزمی، تهران، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and purpose: As one of the antioxidant enzymes, ascorbate peroxidase (APX) has an important role in the defense system of plants against environmental stresses and is effective in regulating the concentration of hydrogen peroxide (H2O2) in plant cells. APX decomposes hydrogen peroxide in order to prevent plant damage. During various environmental stresses, antioxidant enzymes such as APX increase in different plant parts such as fruit. Of course, the rate of biosynthesis of this antioxidant enzyme depends on the intensity and duration of stress in the plant. Considering the importance of the presence of this enzyme in plant structures, this study was conducted to investigate the maximum activity of ascorbate peroxidase enzyme at different levels of surfactants and chaotropes.
Materials and methods: This research was carried out in the research laboratory of Payam Noor University of Kurdistan. In order to prepare the extract from strawberries and blackberries, in the presence of 0.1 M citrate-phosphate buffer with pH 7 and 0.02 phenylmethanesulfonylfluoride solution as a protease inhibitor, the fruits were homogenized separately.
Results: Based on the results of this research, for the ascorbate substrate used in the APX measurement in the extracts prepared from strawberry (ST.APX) and blackberry (BM.APX), the optimal pH is 6.7 and the catalytic efficiency of BM.APX, is about 1.6 times higher than ST.APX was obtained. With the increase of ascorbate concentration in constant concentration of H2O2, the activity of BM.APX and ST.APX increases, so that the highest level of activity of both plant 0. 5 units per mg of protein. A further increase in substrate concentration was associated with substrate inhibition of BM.APX and ST.APX. The activity curve of APX in both plant species is hyperbolic, which follows the Michaelis-Menten kinetics, which indicates the high tendency of the enzyme to consume ascorbate. The activity of ST.APX and BM.APX is inhibited by kojic acid and it is non-competitive in both. Among the used chaotropes and surfactants, only SDS activated the activity of ST.APX and BM.APX, and the activities of ST.APX and BM.APX showed different sensitivities to the surfactants, chaotropes and kojic acid. Despite the fact that BM.APX showed higher activity than ST.APX in the presence of ascorbate and SDS, in the presence of other factors, the rate of inhibition of BM.APX was higher than ST.APX and showed higher percentages of inhibition. Urea and guanidine hydrochloride as reducing agents showed an inhibitory role on ST.APX and BM.APX. The activity of APX The results also showed that the activity of ST.APX and BM.APX was inhibited by kojic acid and it was non-competitive in both. Among the chaotropes and surfactants used, only SDS activated the activity of ST.APX and BM.APX and its maximum effect occurred at a concentration of 0.2 mM. On the other hand, in other factors, the rate of inhibition and reduction of BM.APX activity was higher than ST.APX, and with the exception of ascorbate peroxidase response to kojic acid and sodium calate in strawberry, the enzyme response followed a monophasic decreasing exponential
Conclusion: In this research, ascorbate peroxidase takes a more active form by following Michaelis Menten's kinetics and binding the substrate to the active site of the enzyme, and by performing enzyme catalysis, it causes ascorbate consumption in strawberries and blackberries. Ionic surfactants with an activation mechanism such as SDS and an inhibitory mechanism such as sarkosyl and sodium cholate have different effects on ascorbate peroxidase. Non-ionic surfactants such as kojic acid and chaotrope agents reduce the activity of ascorbate peroxidase in both plant species. The position of kojic acid is different from the position of ascorbate due to the non-competitive type of inhibition.
کلیدواژه‌ها [English]
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