نوع مقاله : پژوهشی
نویسندگان
1 دانشجوی ارشد آگرواکولوژی دانشکده کشاورزی، دانشگاه زابل، زابل، ایران
2 دانشگاه زابل
3 گروه زراعت و اصلاح نباتات پژوهشکده کشاورزی، پژوهشگاه زابل، زابل، ایران.
4 دانشیار گروه زراعت دانشکده کشاورزی، دانشگاه زابل، زابل، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and objectives: the use of biological stimulants in the direction of producing biological products compatible with the environment and in connection with modern agriculture can increase the quantitative and qualitative growth and reduce the effects of environmental stress on them. Cinnamon basil medicinal plant has anticonvulsant, invigorating, tonic, diuretic, digestive booster, anti-bloating, anti-vertigo and anti-vomiting effects. This study was conducted to investigate the physiomorphological and phytochemical responses of Ocimum basilicum to biological stimulants.
Materials and methods: This experiment was carried out as a factorial in the form of a completely random basic design with three replications in the 2022-2033 in greenhouse of the Agricultural Research Institute of Zabol Research Institute located in Chah-Nimeh, Zahak City. Test treatments include foliar spraying with brassinosteroid at 3 levels (0, 200, 400 ppm), carrageenan (0, 150, 300 ppm) and zinc nanoparticles synthesized in ginseng extract. (0, 100, 500 ppm) and were sprayed in stages of 4, 8, and 12 leaves. After one growing season and during the flowering season of the plants, morphological parameters such as plant height, number of inflorescences per plant, number of flowers per inflorescence, fresh and dry weight of the plant were measured. Also, physiological parameters such as photosynthetic pigments (chlorophyll a, chlorophyll b and total chlorophyll) and anthocyanin by spectrophotometric method and phytochemical parameters such as total phenol content by Folin-Ciocalteu's reagent method, total flavonoid by Aluminum chloride colorimetric method, antioxidant activity was measured by DPPH free radical inhibition percentage method. The data were analyzed with SAS statistical software version 9.1 and the average was compared with LSD test at the 5% probability level.
Results: The results showed that plant height, number of inflorescences per plant, number of florets per inflorescence, inflorescence length, plant dry weight, amount of chlorophyll a, b and total, total phenol, total flavonoid and antioxidant activity under the effect of foliar spraying with biological stimulants (in the flowering season) increased significantly. The highest phenolic content (150.49 mg Gallic acid equivalent/gram fresh weight) was accumulated in the foliar treatment with 150 ppm carrageenan + 100 ppm green synthesized nanoparticles. Green zinc nanoparticles had the greatest effect on the antioxidant activity of cinnamon basil extract; So that the highest percentage of inhibition of DPPH free radicals (92.66%) was observed.
Conclusion: In general, it can be said that Brrassinosteroid stimulants, carrageenan, and zinc nanoparticles synthesized in ginseng extract in high concentrations were effective in stimulating vegetative growth and increasing the synthesis of some secondary metabolites.
کلیدواژه‌ها [English]
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