نوع مقاله : پژوهشی
نویسندگان
گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Abstract
Background and Objective: Soil salinity is a major abiotic stress for agriculture in arid and semi-arid regions, significantly affecting seed germination, growth, development, and crop productivity. Quinoa (Chenopodium quinoa Willd.) is a salt-tolerant plant; however, this tolerance varies greatly among different quinoa cultivars. Quinoa is of great interest due to its excellent nutritional properties, such as high protein content and a unique amino acid profile, as well as its high tolerance to abiotic stresses. This makes quinoa an important crop for food security. The fungus Piriformospora indica enhances plant salt tolerance through mechanisms such as increasing the efficiency of water and nutrient uptake, stimulating the antioxidant system, and producing protective compounds and silicon nanoparticles enhane salt tolerance of plants by modulating phsiological and biochemical processes. The aim of the present research was to investigate the effect of inoculating with the symbiotic fungus Piriformospora indica and foliar application of silicon nanoparticles on salt stress tolerance in Giza1 quinoa cultivar.
Materials and Methods: This research was conducted as a factorial experiment based on a completely randomized design with three replications on Giza1 quinoa cultivar. The experimental treatments included three salinity levels (0, 200, and 400 mM NaCl), three levels of silicon nanoparticles (0, 50, and 100 mg/l, 3 times at 10-days interval), and two levels of Piriformospora indica (non-inoculation and inoculation). Biochemical traits including the content of total protein, malondialdehyde, hydrogen peroxide, proline, soluble sugars, free amino acids, antioxidant capacity assessed by the DPPH free radical scavenging method, and the activity of antioxidant enzymes were evaluated.
Findings: Analysis of variance of the data showed that the interaction effect of silicon nanoparticle application and Piriformospora indica inoculation was significant on all biochemical indices studied in the Giza1quinoa cultivar. Although salt stress significantly increased oxidative stress indices (hydrogen peroxide and malondialdehyde), the simultaneous application of silicon nanoparticles and P. indica markedly reduced these indices. Conversely, they increased the content of proline, soluble sugars, free amino acids, and total protein, as well as the activity of antioxidant enzymes (asorbate eroxidase, catalase and peroxidase) and DPPH free radical scavenging capacity.
Conclusion: The results of the present study indicated that the combined application of silicon nanoparticles (especially at 100 mg/L) and Piriformospora indica inoculation was the most effecive treatment for improving the biochemical indices of Giza1 quinoa cultivar under salt stress and also on reducing the destructive effects of this stress.
Keywords: Antioxidant, Physiological indices, Salinity stress, Piriformospora indica
کلیدواژهها [English]
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