نوع مقاله : پژوهشی
نویسندگان
1 گروه باغبانی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2 Horticulture Department, Gorgan University of Agricultural Sciences and Natural Baseij SQ
3 گروه باعبانی، دانشکاه ابوعلی سینا
4 دانشیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Objectives: Soil salinization is one of the major threats to food security and ecosystem health in arid and semi-arid regions worldwide. High salt concentrations cause osmotic imbalance, oxidative damage, and other hazards to plants, leading to reduced growth, reduced biomass, and even complete crop loss. Salt-tolerant plant growth-promoting rhizobacteria (HT-PGPR), as a group of beneficial soil microorganisms, are emerging as a valuable biological fertilizer to reduce the negative effects of high salt concentrations and improve plant growth. This study aimed to investigate the effects of inoculation of two strains of halophilic bacteria isolated from saline plants on reducing the negative effects of salinity in three grape cultivars.
Materials and Methods: This study investigated the potential of two strains of halophilic bacteria Bacillus subtilis POE26 and Oceanobacillus sp.76 to increase tolerance to NaCl salinity stress in three grape cultivars (Fakhri, Bidaneh Sefid and Gaznehei). This research was conducted as a factorial experiment in a completely randomized basic design with three replications. The response of plants to salinity stress and bacterial inoculation was analyzed by evaluating growth parameters and physiological traits.
Results: The results of this study showed that in all the studied cultivars, salinity stress caused a decrease in internode length, leaf area and relative leaf water content, so that in (salinity 0 mM) the maximum values were recorded in these traits, although the Oceanobacillus sp. 76 strain increased the internode length and leaf area compared with the uninoculated control. Stem diameter and number of branches decreased under the influence of salinity stress, and leaf protein and Ion leakage parameters increased. Under salinity stress, it was observed that the activity of both bacterial strains studied increased total soluble sugar and protein and reduced electrolyte leakage. Among the cultivars, Fakhri recorded the highest internode length (3.19cm), leaf area (32.22cm2), lateral branch length (32.50cm) and soluble sugars (117.72mg/gFW).
Conclusion: In this study, the role of two strains of halophilic bacteria Bacillus subtilis POE26 and Oceanobacillus sp.76 isolated from tissues of halophytic plants in reducing salinity stress in three grape cultivars (Fakhri, Bidaneh Sefid and Gaznehei) was evaluated. The results obtained show that the application of both bacterial strains positively improved both morphological and physiological characteristics. Salinity stress reduced growth parameters, relative leaf water content and total soluble sugar and caused an increase in leaf protein and Ion leakage, although bacterial inoculation improved growth parameters and caused an increase in protein, soluble sugars and reduced Ion leakage compared with the uninoculated control.
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کلیدواژهها [English]
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