بررسی اثر پارامتر‌های محیطی بر توانمندی آنتی‌اکسیدانی گیا‌هان دارویی جمع‌آوری شده از منطقه پاوه و اورمانات

نوع مقاله : مقاله کامل علمی پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 دانشگاه رازی کرمانشاه

چکیده

سابقه و هدف: گیاهان دارویی که خودرو در مناطق مختلف کشور یافت می‌شوند که از تنوع بالایی برخوردار بوده و میزان ترکیبات موثره موجود در آنها نیز متغیر است. این تغییرات می‌تواند تابع گونه گیاهی و شرایط محیطی مانند دما، بارش و ارتفاع و یا شرایط خاک باشد. از این رو شناخت گیاهان دارویی یک منطقه می‌تواند نقش مهمی در درک پتانسیل گیاهان دارویی آن منطقه داشته باشد.
مواد و روش‌ها: در این مطالعه تعداد 348 نمونه گیاهی از 116 گونه گیاه دارویی موجود در منطقه‌ی پاوه و اورمانات جمع‌آوری شد. پس از تهیه نمونه هرباریومی گیاهان در شرایط سایه به صورت لایه نازک خشک شدند. از نمونه‌های خشک شده برای اندازه گیری پتانسیل آنتی‌اکسیدانی کل (TAOC) استفاده شد. به علاوه اطلاعات محیطی آنها نیز استخراج و پارامترهای شیمیایی خاک محل آنها نیز جمع‌آوری شد. برای تهیه‌ی معرف TAOC از اسیدسولفوریک (H2SO4) 6/0 مولار، سدیم‌فسفات (Na3PO4) 28 میلی‌مولار و آمونیوم‌ مولیبدات (N6H24Mo7O24) 4 میلی‌مولار استفاده شد. در نهایت رابطه‌ی بین آنها با TAOC با استفاده از مدل‌های رگرسیونی در داده‌کاوی جستجو شد.
یافته‌ها: به منظور مدل‌سازی و تأثیر پارامترهای محیطی بر میزان آنتی‌اکسیدان کل گیاهان دارویی در منطقه مطالعاتی، ابتدا همبستگی بین آنها بررسی شد. نتایج نشان داد که بین پارامترهای محیطی با TAOC همبستگی قابل قبولی وجود ندارد. به طوری که بالاترین همبستگی با هدایت الکتریکی و برابر با 25/0 بدست آمد. در بررسی داده ها، روابط رگرسیونی دو تا چند متغیره نتایج رضایت بخشی را نشان نداد، بطوری که در روش گام به گام حداکثر 31 درصد همبستگی به دست آمد، اما مدل رگرسیون درختی M5 با تفکیک داده‌ها به 29 فضا، مقادیر TAOC را با 91 درصد همبستگی و 95/0RMSE= میلی‌گرم بر گرم ماده خشک با 6/16درصد خطا برآورد نمود.
نتیجه‌گیری: نتایج بررسی ارتباط میزان آنتی‌اکسیدان کل (TAOC) گیاهان دارویی با شرایط محیطی و خاک به دست آمد نشان داد که میزان توانمندی آنتی‌اکسیدانی گیاه بر اساس آنتی‌اکسیدان کل به تنهایی هیچ همبستگی معنی‌داری با شرایط محیطی و خاک ندارد. به‌طوری که حتی رابطه چند متغیره آن نیز همبستگی معنی‌داری را نشان نداده که می‌تواند ناشی از تنوع بالای در گونه گیاهان مورد مطالعه باشد. بنا بر این در کنار توانمندی ژنتیکی، میزان فعالیت آنتی‌اکسیدانی کل در گیاهان دارویی به شرایط محیطی و اقلیمی نیز وابسته است. از طرفی در درک بهتر پتانسیل دارویی گیاهان دارویی با پارامترهای چندگانه محیطی و ژنتیکی استفاده از روش‌های داده کاوی از اهمیت زیادی برخوردار است. نتایج تحقیق حاضر نشان داد که روش درخت تصمیم M5 روشی کارامد در این زمینه است.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of environmental parameters effect on the antioxidant capacity of medicinal plants

نویسندگان [English]

  • Azim Ghasemnezhad 1
  • Mohammad Sanei 1
  • Khalil Ghorbani 1
  • Mohammad Masoumi 2
  • Alireza Sadeghi Mahonak 2
1 Gorgan University of Agricultural Sciences and Natural Resources
2 Razi University of Kermanshah
چکیده [English]

Background: Medicinal plants have wide diversity and the amounts of active compounds of them are highly variable in different areas of Iran. These changes can be depending on the plant species and environmental conditions such as temperature, precipitation and elevation or soil conditions. Therefore, evaluation the medicinal plants of an area can play an important role in understanding the potential of medicinal plants.
Materials and Methods: In this experiment, 348 plant samples were collected from 116 medicinal plants in Paveh and Ormanat. The samples were tested after identification in herbarium laboratory of Razi University of Kermanshah and were transferred to the laboratory of Department of Horticultural Sciences of Gorgan University of Agricultural Sciences and Natural Resources for antioxidant potency analysis. After preparation the herbarium samples, the plants were dried in shade as thin layer. The dried samples were employed to measure total antioxidant potential (TAOC) via TAOC method. Furthermore, climate data and soil chemical parameters were collected. To prepare the TAOC reagent, 0.6 mM sulfuric acid (H2SO4), 28 mM sodium phosphate (Na3PO4) and 4 mM ammonium molibdat (N6H24Mo7O24) were used. Finally, the relationship between them and TAOC was evaluated using regression models by data mining.
Finding: In order to assess the effect of environmental parameters on the total antioxidant content of medicinal plants in this study, the correlation between data was evaluated. The results showed that there is no correlation between environmental parameters and TAOC. In data analysis, the two-variable regression equations did not show satisfactory results, as in the stepwise method, a maximum of 31% correlation was observed, but the M5 tree regression model by dividing the data into 29 spaces provided the TAOC values which showed 91% correlation and RMSE = 16.6%.
Conclusion
The results of our study showed that, the amount of antioxidant capacity of plant based on total antioxidant had no significant correlation with environmental and soil conditions. Moreover, its multivariate relationship did not show a significant correlation, which could be due to the high diversity in the studied species. It concluded that in addition to genetic potential, total antioxidant activity of medicinal plants depends on the environmental and climatic conditions. However, understanding the potential of medicinal plants with multiple environmental and genetic parameters using data mining techniques is an important trait. Overall, the results of this study showed that,in present study the M5 decision tree method was an efficient method in evaluation of antioxidant capacity of plant.

کلیدواژه‌ها [English]

  • Antioxidant
  • Oramanat
  • Environmental parameter
  • M5 decision tree
  • TAOC
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