نوع مقاله : مقاله کامل علمی پژوهشی
نویسندگان
1 نویسنده مسئول، محقق و دکتری بیماری‌شناسی گیاهی، بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، گرگان، ایران
2 استاد بخش تحقیقات ویروس‌شناسی، مؤسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Introduction: Soybean is a plant belonging to the Fabaceae family and is one of the most important oil seed in the world. The northern provinces of Iran are the main soybean cultivation regions in the country, but over the years, soybean podding disorder has reduced yield in this regions and caused up to 100% damage. The disorder is more severe in late planting farms, and symptoms can be observed as plant greening, accumulation of flowers and pods, abnormal pods, bud blight, and lack of seed in pod. The results of previous studies have shown the possible role of viruses in occurrence of soybean disorder. This research was conducted to investigate the Nepovirus effect on soybean yield and cultivars, and possible role on soybean podding disorder.
Materials and Methods: 770 soybean sample from Golestan provinces were collected befor and after disorder appeared, and tested by DAS-ELISA and RT-PCR test for investigate the possible role of Nepovirus in disorder. The response of soybean cultivars to Nepovirus and soybean podding disorder, was evaluated in greenhouse and natural conditions. In greenhouse, 2 soybean cultivars (Katol, Saman) were inoculated mechanically at the 4-6 leaf stage. For comparison of soybean cultivars response to studied viruses and evaluation of possible role of Nepovirus on soybean podding disorder in natural condition, the experiment was performed in Golestan Agricuitural Research center as a split plot design with three factors: using net, virus inoculation and soybean cultivars with 3 replications in two years. Nepoviruses inoculated on specific plots on soybean plants at 4-6 leaf stages. ELISA and RT-PCR tests were used to ensure the plants infection with inoculated viruses, and growth indices were measured at physiological growth stage. Data were analyzed by two-way ANOVA, and the means were compared with LSD test at 5% confidence level, using SPSS software version 16.0.
Results and Discussion: Comparing of viruse frequency in not disorder plants with podding disorder plants, did not show significant relationship between viruses and disorder incidence. In greenhouse, virus inoculation on soybean plants, caused chlorosis, stunt and systemic necrosis, but the typical symptoms of disorder such as severe falling of flowers, re-flowering, and plant greening did not show. In natural conditions, disorder was observed in all soybean cultivars, but disorder incidence was different among cultivars, and Williams variety showed less disorder in four treatments. The highest and lowest plant growth indices were observed in non-inoculation virus with use of net treatment and, virus inoculation with no use of net, respectively. Mechanical virus inoculation on different soybean cultivars, although reduced growth indices and soybeans yield, but had no significant effect on the podding disorder. In treatments with not controlling of sucker pests, the incidence of disorder was significantly higher than other treatments.
Conclusion: Virus inoculation on soybean plants in greenhouse and natural conditions did not cause podding disorder syndrome. Viruses in soybean plants, reduce growth indices and aggravate disorder due to the stress they inflict on the soybean plant, but are not probably the cause of the disorder alone. In treatments with not controlling of sucker pests, disorder incidence was significantly higher, so the role of sucker pests was estimated effective and important.
کلیدواژه‌ها [English]
)