نوع مقاله : پژوهشی
نویسندگان
1 دانشکده کشاورزی و منابع طبیعی، دانشگاه هرمزگان، بندرعباس، ایران
2 Iran, BandarAbbas, Hormozgan, University of Hormozgan, Faculty of Agriculture and Natural Resource,
3 گروه کشاورزی، گروه مهندسی تولید و اصلاح نباتات، مرکز آموزش عالی میناب، دانشگاه هرمزگان، بندرعباس، ایران.
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and objectives: Due to the extent of salinity in Iranian soils, the high salinity of water and soil can limit crop quality and productivity. On the other hand, due to the increasing global demand for the use of medicinal plants such as peppermint, today the use of sodium nitroprusside is one of the new strategies to improve and increase the yield of plants in adverse environmental conditions such as water and soil salinity. Therefore, the aim of this study was to evaluate the effect of sodium nitroprusside (SNP) on the morphophysiological characteristics of peppermint (Menthapiperita L.) under salinity stress.
Materials and Methods: This experiment was performed in the greenhouse of Hormozgan University as a factorial in a completely randomized design with three replications in 1400. In this study, two factors of sodium nitroprusside (0, 0.1, and 0.2 mmol) and salinity levels (0, 25, 50, 75, and 100 mmol) were studied. Rhizomes 4 cm long were selected and planted in pots with a diameter of 20 cm and a height of 18 cm. The soil in each pot was a mixture of arable soil, sand, and rotted manure in a ratio of 1: 3: 6 (this ratio was suitable for growing peppermint rhizomes). For each treatment, 3 pots were considered as 3 replications. After plant growth, the leaves were sprayed with 200 ml of sodium nitroprusside solution. Control plants were sprayed using distilled water. Foliar application was performed with an interval of three days at a rate of 10 ml per pot and once every three days. 24 hours after the last foliar application and two months after planting, the plants were treated with salinity. Twenty days after application of stress, plants were harvested and traits were evaluated. Measured variables included Leaf length and width, stem diameter, leaf wet and dry weight, chlorophyll a, chlorophyll b, carotenoid, ion leakage, proline, catalase and oxidative capacity.
Results: The results showed that the presence of 0.2 mM sodium nitroprusside compared to its absence caused a significant increase in leaf length, leaf width, chlorophyll a, b, carotenoid and proline to the extent of (80.60, 65.38, 150.70 38, 120 and 101.13 percent) in 100 mM salt stress. While it was observed that the use of 0.1 mM sodium nitroprusside decreased ion leakage by 365.90% at 100 mM salinity stress compared to the control.
Conclusion: Therefore, according to the results of the present research, it is possible to recommend foliar spraying of sodium nitroprusside to reduce the negative effects of salinity stress in peppermint plants.
کلیدواژهها [English]